Invitrogen

ENO1 Polyclonal Antibody

ENO1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

ENO1 Antibody (PA5-21387) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of ENO1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ENO1 Polyclonal Antibody (Product # PA5-21387) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

ENO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ENO1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

ENO1 Antibody — Figure 5 Validation of expression profiling of proteins by Western blotting. Four of the identified proteins, PRDX1 ( A ), GLRX3 ( B ), ENO1 ( C ), and CASP1 ( D ), responding to AGE and/or FruArg treatment in LPS-stimulated BV-2 cells were validated using Western blotting. Protein intensities were quantified by Image J software, normalized to beta-actin, and expressed as percentage of untreated controls. Data are means +- SEM (n>=5); #, P<0.05, untreated vs. LPS; *, P<0.05, LPS vs. LPS+AGE; **, P<0.05, LPS vs. LPS+FruArg. {TM}

产品信息

PA5-21387

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:20,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

点杂交 (DB)

Assay-dependent

产品规格

种属反应

Human, Mouse

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 199 and 434 of ENO1 (Uniprot ID#P06733)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

0.1M tris glycine, pH 7, with 10% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_11154109

产品详细信息

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse brain.

Predicted reactivity: Mouse (94%), Rat (93%), Xenopus laevis (90%), Chicken (93%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (93%), Guinea pig (95%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Enolase (2-phosphogly-cerate hydrolyase or phosphopyruvate hydrates) is a glycolytic enzyme that catalyzes the dehydration and conversion of 2-phosphoglycerate to phosphoenolpyruvate. It comprises three distinct subunits, alpha, beta and gamma. Non Neuronal Enolase(ENO1) is an isoform of mammalian enolase and is composed of 2 alpha subunits. The gene for ENO1 also encodes a shorter monomeric structural lens protein, tau-crystallin. Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.Used as a diagnostic marker for many tumors and, in the heterodimeric form, alpha/gamma, as a marker for hypoxic brain injury after cardiac arrest. Also marker for endometriosis. Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies. Is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidism.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Quantitative Proteomic Analysis of Differentially Expressed Protein Profiles Involved in Pancreatic Ductal Adenocarcinoma.

Pancreas

Kuo KK,Kuo CJ,Chiu CY,Liang SS,Huang CH,Chi SW,Tsai KB,Chen CY,Hsi E,Cheng KH,Chiou SH

Published figure using ENO1 polyclonal antibody (Product # PA5-21387) in Immunohistochemistry

Fri Jan 01 00:00:00 UTC 2016

Quantitative Proteomic Analysis of Differentially Expressed Protein Profiles Involved in Pancreatic Ductal Adenocarcinoma.

Pancreas

Kuo KK,Kuo CJ,Chiu CY,Liang SS,Huang CH,Chi SW,Tsai KB,Chen CY,Hsi E,Cheng KH,Chiou SH

Published figure using ENO1 polyclonal antibody (Product # PA5-21387) in Immunohistochemistry

Fri Jan 01 00:00:00 UTC 2016

Proteomic analysis of the effects of aged garlic extract and its FruArg component on lipopolysaccharide-induced neuroinflammatory response in microglial cells.

PloS one

Zhou H,Qu Z,Mossine VV,Nknolise DL,Li J,Chen Z,Cheng J,Greenlief CM,Mawhinney TP,Brown PN,Fritsche KL,Hannink M,Lubahn DB,Sun GY,Gu Z

PA5-21387 was used in western blot to study the effects of aged garlic extract and its FruArg component by proteomic analysis and lipopolysaccharide-induced neuroinflammatory response in microglial cells

Tue Jan 26 00:00:00 UTC 2016

生物信息学

蛋白别名: 2-phospho-D-glycerate hydro-lyase; 2-phospho-D-glycerate hydrolase; alpha enolase like 1; Alpha-enolase; c-Myc promoter binding protein; C-myc promoter-binding protein; enoa; Enolase 1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; MBP-1; MPB-1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; tau-crystallin

查看更多收起

基因别名: 0610008I15; AL022784; Eno-1; ENO1; ENO1L1; HEL-S-17; MBP-1; MBPB1; MPB1; NNE; PPH

查看更多收起

UniProt ID: (Human) P06733, (Mouse) P17182

查看更多收起

Entrez Gene ID: (Human) 2023, (Mouse) 13806

查看更多收起

功能