Invitrogen

EPB41L5 Polyclonal Antibody

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2 篇参考文献

查看其他5个EPB41L5抗体

抗体检测数据 (6)
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EPB41L5 Antibody in Immunocytochemistry (ICC/IF)

EPB41L5 Antibody in Immunocytochemistry (ICC/IF)

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EPB41L5 Antibody (PA5-58009) in ICC/IF

Immunofluorecent analysis of EPB41L5 in human cell line U-2 OS using EPB41L5 Polyclonal Antibody (Product # PA5-58009). Staining shows localization to plasma membrane.

EPB41L5 Antibody in Immunocytochemistry (ICC/IF)

EPB41L5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EPB41L5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EPB41L5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EPB41L5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EPB41L5 Antibody in Western Blot (WB)

EPB41L5 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

EPB41L5 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

EPB41L5 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

EPB41L5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorecent analysis of EPB41L5 in human cell line U-2 OS using EPB41L5 Polyclonal Antibody (Product # PA5-58009). Staining shows localization to plasma membrane.

EPB41L5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of EPB41L5 in human testis using EPB41L5 Polyclonal Antibody (Product # PA5-58009) shows strong cytoplasmic positivity in cells in seminiferous ducts.

EPB41L5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of EPB41L5 in human kidney using EPB41L5 Polyclonal Antibody (Product # PA5-58009) shows strong cytoplasmic positivity in cells in glomeruli.

EPB41L5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of EPB41L5 in human skeletal muscle using EPB41L5 Polyclonal Antibody (Product # PA5-58009) shows very weak positivity in myocytes.

EPB41L5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of EPB41L5 in human liver using EPB41L5 Polyclonal Antibody (Product # PA5-58009) shows weak cytoplasmic positivity in hepatocytes.

EPB41L5 Antibody in Western Blot (WB) — Western blot analysis of EPB41L5 in control (vector only transfected HEK293T lysate) and EPB41L5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a EPB41L5 Polyclonal Antibody (Product # PA5-58009).

EPB41L5 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 3 ZBTB7A negatively regulates EPB41L5. a The Venn diagram shows an overlap between GSEA results. b Heatmap of the expression and enrichment of 18 overlapping genes in the GSEA results. c , d mRNA expression levels of 18 overlapping genes in shZBTB7A U87 cells and pCDH ZBTB7A U343 cells. e Negative correlation of the gene expression levels of MDK , IL33 , and EPB41L5 with ZBTB7A (GlioVis). f , g mRNA expression levels of MDK , IL33 , and EPB41L5 in shZBTB7A U87 cells and pCDH ZBTB7A U343 cells. h , i Western blot analysis of ZBTB7A and EPB41L5 in shZBTB7A U87 cells and pCDH ZBTB7A LN229 and U343 cells. j Western blot analysis of ZBTB7A and EPB41L5 in GBM cell lines. k EPB41L5 IHC staining in increasing grades of glioma using TMA blots. l IHC analysis of EPB41L5 expression in TMA blots. NB, n = 9; Grades I-II, n = 30; III-IV, n = 28. Scale bars: 50 mum. * p < 0.05, ** p < 0.01, *** p < 0.001.

EPB41L5 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 6 EPB41L5 induces EMT and increases tumorigenesis. a The transcriptional activity of the EPB41L5 promoter with deletion of the #3 position in shZBTB7A U87 cells was investigated by luciferase assay. b mRNA levels of ZBTB7A and EPB41L5 in shZBTB7A#4 U87 cells. c To measure the ability of ZBTB7A R399L to repress promoter activity when ZBTB7A protein expression was low, we performed a luciferase assay in shZBTB7A#4 U87 cells. d The -130/-56 region was amplified with ChIP primers, showing the amount of DNA precipitation by ZBTB7A antibody or IgG by cotransfection of Myc ZBTB7A and EPB41L5 promoters in shZBTB7A#4 U87 cells. e Schematic of the experimental procedure of orthotopic GBM nude mouse models injected with shZBTB7A#4 U87 cells and Western blot analysis of ZBTB7A and EPB41L5 in tumor samples ( n = 4 mice/group). f H&E staining and IHC staining of anti-ZBTB7A, EPB41L5, and EMT markers (N-cadherin, beta-catenin, and vimentin) in orthotopic GBM nude mouse models injected with shZBTB7A#4 U87 cells. g , h Expression of EMT markers by Western blot and qPCR analyses in shZBTB7A U87 cells. Scale bars: 20 mum. n.s; not significant, * p < 0.05, ** p < 0.01, *** p < 0.001.

EPB41L5 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Fig. 4 ZBTB7A expression regulates tumorigenesis in GBM cells. a Western blot analysis of ZBTB7A expression in pCDH ZBTB7A LN229 and U343 cells. b Migration assay of pCDH ZBTB7A LN229 and U343 cells after 24 h. c Transwell invasion assay of pCDH ZBTB7A LN229 and U343 cells after 48 h. d Effect of ZBTB7A overexpression on colony formation in pCDH ZBTB7A LN229 and U343 cells. e Schematic diagram and photograph of a subcutaneous tumor experiment using nude mice. f Western blot analysis of ZBTB7A and EPB41L5 in pCDH ZBTB7A U343 subcutaneous tumors. g Tumor volume was calculated according to the formula at 2-3 d intervals for 5 wk ( n = 5 mice/group). h The weight of each tumor was measured ( n = 8 mice/group). i IHC analysis of pCDH ZBTB7A U343 subcutaneous tumors using an IHC profiler ( n = 5 mice/group). j IHC staining using anti-ZBTB7A, anti-EPB41L5, and anti-Ki67 antibodies and H&E staining. Scale bars: 50 mum, 20 mum. * p < 0.05, ** p < 0.01, *** p < 0.001.

产品信息

PA5-58009

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:200-1:500

-

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

-

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human EPB41L5. Recombinant protein control fragment (Product #RP-102775).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2641063

产品详细信息

Immunogen sequence: DLLEATIGDV IGASDTMETS QALNDVNVAT RLPGLGEPEV EYETLKDTSE KLKQLEMENS PLLSPRSNID VNINSQEEVV KLTEKC

Highest antigen sequence identity to the following orthologs: Mouse - 60%, Rat - 60%.

靶标信息

May contribute to the correct positioning of tight junctions during the establishment of polarity in epithelial cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ZBTB7A suppresses glioblastoma tumorigenesis through the transcriptional repression of EPB41L5.

Experimental & molecular medicine

Jeong JH,Park SH,Kim H,Nam HY,Kim SH,Jeong M,Kong MJ,Son J,Jeong JE,Song JH,Kim SW,Choi KC

Published figure using EPB41L5 polyclonal antibody (Product # PA5-58009) in Western Blot

Sun Jan 01 00:00:00 UTC 2023

ZBTB7A suppresses glioblastoma tumorigenesis through the transcriptional repression of EPB41L5.

Experimental & molecular medicine

Jeong JH,Park SH,Kim H,Nam HY,Kim SH,Jeong M,Kong MJ,Son J,Jeong JE,Song JH,Kim SW,Choi KC

Published figure using EPB41L5 polyclonal antibody (Product # PA5-58009) in Western Blot

Sun Jan 01 00:00:00 UTC 2023

ZBTB7A suppresses glioblastoma tumorigenesis through the transcriptional repression of EPB41L5.

Experimental & molecular medicine

Jeong JH,Park SH,Kim H,Nam HY,Kim SH,Jeong M,Kong MJ,Son J,Jeong JE,Song JH,Kim SW,Choi KC

Published figure using EPB41L5 polyclonal antibody (Product # PA5-58009) in Western Blot

Sun Jan 01 00:00:00 UTC 2023

生物信息学

蛋白别名: Band 4.1-like protein 5; Erythrocyte membrane protein band 4.1-like 5; yurt homolog

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基因别名: BE37; EPB41L5; KIAA1548; YMO1; YRT

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UniProt ID: (Human) Q9HCM4

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Entrez Gene ID: (Human) 57669

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cytoskeletal protein binding protein domain specific binding

in utero embryonic development epithelial to mesenchymal transition neural plate morphogenesis apical constriction substrate-dependent cell migration, cell attachment to substrate ectoderm development endoderm development mesoderm migration involved in gastrulation unidimensional cell growth posttranscriptional regulation of gene expression positive regulation of epithelial cell migration positive regulation of epithelial to mesenchymal transition negative regulation of cell-cell adhesion actomyosin structure organization negative regulation of protein binding positive regulation of protein binding somite rostral/caudal axis specification axial mesoderm morphogenesis paraxial mesoderm development embryonic foregut morphogenesis positive regulation of focal adhesion assembly regulation of establishment of protein localization left/right axis specification cellular response to transforming growth factor beta stimulus