Invitrogen

EPS8 Polyclonal Antibody

EPS8 Antibody in Immunocytochemistry (ICC/IF)

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EPS8 Antibody (PA5-18196) in ICC/IF

Immunofluorescence analysis of EPS8 was performed using 70% confluent log phase RAW 264.7 cells treated with LPS (100 ng/mL, 24h). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with EPS8 Polyclonal Antibody (Product # PA5-18196) at 5 microgram/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor®... View More

EPS8 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-18196

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

免疫组化（石蜡） (IHC (P))

6-8 µg/mL

免疫细胞化学 (ICC/IF)

5 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (SGVESFDEGSSH) corresponding to the C-terminus amino acids of EPS8 (aa 811-822).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_10988198

产品详细信息

This antibody is predicted to react with canine, human, mouse, porcine and rat based on sequence homology.

This antibody is tested in Peptide ELISA: antibody detection limit dilution 8,000.

靶标信息

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

仅用于科研。不用于诊断过程。未经明确授权不得转售。