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ERI1 Antibody (PA5-102031) in ICC/IF
Immunocytochemistry analysis of ERI1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ERI1 polyclonal antibody (Product # PA5-102031) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-102031
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ERI1(Accession Q8IV48), corresponding to amino acid residues F267-R317.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2851462
Product Specific Information
Antibody detects endogenous levels of total ERI1.
Target Information
RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3 ' exoribonuclease; 3' exoribonuclease; 3'-5' exonuclease ERI1; 3'-5' exoribonuclease 1; enhanced RNAi protein 1; enhanced RNAi three prime mRNA exonuclease homolog 1; Eri-1 homolog; HEXO; histone mRNA 3' end-specific exonuclease; Histone mRNA 3'-end-specific exoribonuclease; Histone mRNA 3'-exonuclease 1; protei; Protein 3'hExo; three prime histone mRNA exonuclease 1; three prime mRNA exonuclease 1
Gene Aliases: 3'EXO; 3'HEXO; 3110010F15Rik; eri-1; ERI1; HEXO; RGD1308378; THEX1
UniProt ID: (Human) Q8IV48, (Rat) Q5FVR4, (Mouse) Q7TMF2
Entrez Gene ID: (Human) 90459, (Rat) 361159, (Mouse) 67276
3'-5' exonuclease activity
rRNA binding
ribosome binding
metal ion binding
histone pre-mRNA stem-loop binding
3'-5'-exoribonuclease activity
nucleic acid binding
RNA binding
nuclease activity
exonuclease activity
hydrolase activity
phosphoric ester hydrolase activity
T/G mismatch-specific endonuclease activity
retroviral 3' processing activity
exoribonuclease
exonucleolytic trimming to generate mature 3'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
gene silencing by RNA
rRNA 3'-end processing
histone mRNA catabolic process
DNA catabolic process, exonucleolytic
rRNA processing
nucleic acid phosphodiester bond hydrolysis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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