Invitrogen

ERK2 Polyclonal Antibody

查看其他45个ERK2抗体

抗体检测数据 (2)

ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ERK2 Antibody (PA5-119548) in IHC (P)

Immunohistochemistry of ERK2 in paraffin-embedded mouse lung. Sample was treated for antigen retrieval with Citrate buffer (pH 6.0, 15 min) and incubated with polyclonal ERK2 antibody (Product # PA5-119548) at a dilution of 1:500.

ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ERK2 Antibody in Western Blot (WB)

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ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of ERK2 in paraffin-embedded mouse lung. Sample was treated for antigen retrieval with Citrate buffer (pH 6.0, 15 min) and incubated with polyclonal ERK2 antibody (Product # PA5-119548) at a dilution of 1:500.

ERK2 Antibody in Western Blot (WB) — Western blot analysis of ERK2 in various whole cell extracts (30 µg). Sample was separated with 10% SDS-PAGE, incubated with polyclonal ERK2 antibody (Product # PA5-119548) at a dilution of 1:1000, and followed by HRP-conjugated anti-rabbit IgG.

产品信息

PA5-119548

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

-

免疫组化（石蜡） (IHC (P))

Assay-dependent

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ERK2.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2913121

产品详细信息

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

靶标信息

MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: ERK-2; ERT1; Extracellular signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p42-MAPK; protein tyrosine kinase ERK2

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基因别名: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-2; ERK2; ERT1; Mapk; MAPK1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2

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UniProt ID: (Human) P28482, (Mouse) P63085

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Entrez Gene ID: (Human) 5594, (Mouse) 26413

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phosphotyrosine binding DNA binding protein serine/threonine kinase activity MAP kinase activity protein binding ATP binding transcription factor binding RNA polymerase II carboxy-terminal domain kinase activity kinase activity phosphatase binding mitogen-activated protein kinase kinase kinase binding identical protein binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding non-receptor serine/threonine protein kinase

MAPK cascade activation of MAPK activity MAPK import into nucleus transcription, DNA-templated protein phosphorylation apoptotic process chemotaxis response to stress cellular response to DNA damage stimulus cell cycle signal transduction chemical synaptic transmission axon guidance positive regulation of cell proliferation fibroblast growth factor receptor signaling pathway response to toxic substance positive regulation of peptidyl-threonine phosphorylation regulation of phosphatidylinositol 3-kinase signaling viral process peptidyl-serine phosphorylation peptidyl-threonine phosphorylation sensory perception of pain cytosine metabolic process platelet activation positive regulation of cell migration thyroid gland development regulation of protein stability lipopolysaccharide-mediated signaling pathway positive regulation of telomere maintenance via telomerase regulation of stress-activated MAPK cascade mammary gland epithelial cell proliferation Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB signaling pathway outer ear morphogenesis response to exogenous dsRNA response to estrogen negative regulation of cell differentiation positive regulation of translation positive regulation of transcription, DNA-templated thymus development T cell receptor signaling pathway B cell receptor signaling pathway regulation of sequence-specific DNA binding transcription factor activity regulation of cytoskeleton organization positive regulation of telomerase activity Bergmann glial cell differentiation long-term synaptic potentiation face development JAK-STAT cascade involved in growth hormone signaling pathway lung morphogenesis trachea formation labyrinthine layer blood vessel development cardiac neural crest cell development involved in heart development ERK1 and ERK2 cascade response to epidermal growth factor caveolin-mediated endocytosis regulation of Golgi inheritance cellular response to granulocyte macrophage colony-stimulating factor stimulus regulation of cellular response to heat positive regulation of telomere capping regulation of early endosome to late endosome transport organ morphogenesis phosphorylation response to lipopolysaccharide intracellular signal transduction cellular response to organic substance