Invitrogen

ERK2 Recombinant Rabbit Monoclonal Antibody (SZ25-01)

ERK2 Antibody in Immunocytochemistry (ICC/IF)

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ERK2 Antibody (MA5-32025) in ICC/IF

Immunofluorescence of MAPK1 was performed using HeLa wild-type and MAPK1 KO cells that were transfected with a green or a far-red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) or methanol for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the MAPK1 recombinant monoc... View More

ERK2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ERK2 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Loss of signal in immunofluorescence was observed for target protein in MAPK1 knockout (KO) cell line using Anti-MAPK1 recombinant monoclonal antibody (Product # MA5-32025). Parental cells were transfected with CellTracker green (Product # C2925) and MAPK1 KO with CellTracker Deep Red (Product # C34565). Data courtesy of YCharOS Inc., an open science company with the mission of characterizing commercially available antibodies using knockout validation. {KO}

产品信息

MA5-32025

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:200-1:1,000

免疫细胞化学 (ICC/IF)

1:50-1:200

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

SZ25-01

抗原

Synthetic peptide within Human ERK2 aa 311-360

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809319

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

MAPK1 (ERK2) is a serine/threonine kinase that acts as an important activator of p90 RSK, MSK, ELK1, and Stat3, and is involved in the MAPK pathway. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: ERK-2; ERT1; Extracellular signal-regulated kinase 2; extracellular-signal-regulated kinase 2; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; mitogen activated protein kinase 1; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; p42-MAPK; protein tyrosine kinase ERK2

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基因别名: 9030612K14Rik; AA407128; AU018647; C78273; ERK; ERK-2; ERK2; ERT1; Mapk; MAPK1; MAPK2; p38; p40; p41; p41mapk; p42-MAPK; P42MAPK; PRKM1; PRKM2

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UniProt ID: (Human) P28482, (Mouse) P63085, (Rat) P63086

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Entrez Gene ID: (Human) 5594, (Mouse) 26413, (Rat) 116590

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