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ERK5 Antibody (MA5-35422) in WB
Western blot analysis of ERK5 in extracts of various cell lines. Samples were incubated with ERK5 Monoclonal antibody (Product # MA5-35422) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3 min.
产品信息
MA5-35422
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
ARC0871
抗原
A synthetic peptide corresponding to a sequence within amino acids 717-816 of human ERK5 (Q13164)
偶联物
形式
Liquid
浓度
0.8 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2849323
产品详细信息
Immunogen sequence: LSKSQVEDPL PPVFSGTPKG SGAGYGVGFD LEEFLNQSFD MGVADGPQDG QADSASLSAS LLADWLEGHG MNPADIESLQ REIQMDSPML LADLPDLQDP
靶标信息
MAPK7 (ERK5) serine/threonine kinase is a member of the MAP kinase family and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK7 is activated by mitogen-activated protein kinase 5 (MAP2K5/MEK5) and is involved in downstream signaling processes of various receptors. In response to extracelluar signals, MAPK7 translocates to the cell nucleus where it regulates gene expression by phosphorylating and activating different transcription factors. Gene deletion of MAPK7 in mice results in defective blood vessel and cardiac development leading to embryonic lethality. MAPK7 has been shown to be critical for endothelial function and maintenance of blood vessel integrity. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. ERK5 is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Big MAP kinase 1; BMK-1; BMK1 kinase; BNhp-1; BNhp1 kinase; ERK-5; extracellular signal regulated kinase 5; Extracellular signal-regulated kinase 5; extracellular-signal-regulated kinase 5; MAP kinase 7; MAPK 7; mitogen activated protein kinase 7; Mitogen-activated protein kinase 7
基因别名: BMK-1; BMK1; ERK-5; ERK4; ERK5; Erk5-T; MAPK7; PRKM7
UniProt ID: (Human) Q13164, (Mouse) Q9WVS8
Entrez Gene ID: (Human) 5598, (Mouse) 23939, (Rat) 114509
protein serine/threonine kinase activity
MAP kinase activity
protein binding
ATP binding
mitogen-activated protein kinase binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
translation factor
translation initiation factor
translational protein
MAPK cascade
cell cycle
signal transduction
peptidyl-serine phosphorylation
cAMP-mediated signaling
cell differentiation
negative regulation of cAMP catabolic process
negative regulation of heterotypic cell-cell adhesion
positive regulation of transcription from RNA polymerase II promoter in response to stress
negative regulation of MAP kinase activity
regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
negative regulation of inflammatory response
positive regulation of protein metabolic process
negative regulation of cyclic-nucleotide phosphodiesterase activity
negative regulation of NFAT protein import into nucleus
negative regulation of response to cytokine stimulus
cellular response to hydrogen peroxide
negative regulation of ERK5 cascade
cellular response to growth factor stimulus
cellular response to laminar fluid shear stress
cellular response to transforming growth factor beta stimulus
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
negative regulation of endothelial cell apoptotic process
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
protein phosphorylation
phosphorylation
negative regulation of apoptotic process
protein autophosphorylation
ERK5 cascade
