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ERN1/ERN2 Antibody (PA5-115434) in ICC/IF
Immunocytochemistry analysis of ERN1/ERN2 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115434) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat a... View More
产品信息
PA5-115434
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human ERN1(Accession O75460), corresponding to amino acid residues P603-V653.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900070
产品详细信息
Antibody detects endogenous levels of total IRE1.
靶标信息
ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1) is a Protein Coding gene. Diseases associated with ERN1 include Arrhythmogenic Right Ventricular Dysplasia, Familial, 5 and Epiphyseal Dysplasia, Multiple, With Early-Onset Diabetes Mellitus. Among its related pathways are Spinocerebellar ataxia and Photodynamic therapy-induced unfolded protein response. Gene Ontology (GO) annotations related to this gene include protein homodimerization activity and transferase activity, transferring phosphorus-containing groups. An important paralog of this gene is ERN2. This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: endoplasmic reticulum (ER) to nucleus signalling 1; endoplasmic reticulum (ER) to nucleus signalling 2; endoplasmic reticulum to nucleus signalling 2; Endoplasmic reticulum-to-nucleus signaling 1; Endoplasmic reticulum-to-nucleus signaling 2; ER to nucleus signalling 1; ER to nucleus signalling 2; ERN1; ERN2; hIRE1p; hIRE2p; inositol-requiring 1; inositol-requiring 1 (Yeast homologue); inositol-requiring 1 alpha; inositol-requiring 1 beta; inositol-requiring enzyme 1; Inositol-requiring protein 1; Inositol-requiring protein 2; IRE1; IRE1 beta; IRE1, S. cerevisiae, homolog of; Ire1-alpha; Ire1-beta; IRE1a; IRE1b; IRE2; mIre1; protein kinase/endoribonuclease; Serine/threonine-protein kinase/endoribonuclease IRE1; Serine/threonine-protein kinase/endoribonuclease IRE2
基因别名: 9030414B18Rik; AI225830; C85377; ERN1; ERN2; hIRE1p; hIRE2p; IRE1; IRE1-BETA; IRE1a; Ire1alpha; IRE1b; IRE1P; IRE2; IRE2p; RGD1559716
UniProt ID: (Human) Q59EE2, (Human) Q76MJ5, (Mouse) Q9EQY0, (Mouse) Q9Z2E3
Entrez Gene ID: (Human) 2081, (Human) 10595, (Mouse) 78943, (Rat) 498013, (Mouse) 26918, (Rat) 365363
magnesium ion binding
endoribonuclease activity
protein serine/threonine kinase activity
protein binding
ATP binding
enzyme binding
Hsp70 protein binding
identical protein binding
protein homodimerization activity
ADP binding
Hsp90 protein binding
endonuclease activity
ribonuclease activity
nucleotide binding
catalytic activity
protein kinase activity
kinase activity
bidentate ribonuclease III activity
transferase activity
transferase activity, transferring phosphorus-containing groups
hydrolase activity
metal ion binding
endoribonuclease
endothelial cell proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
mRNA cleavage
mRNA catabolic process
protein phosphorylation
activation of signaling protein activity involved in unfolded protein response
cell cycle arrest
activation of JUN kinase activity
regulation of macroautophagy
positive regulation of RNA splicing
response to endoplasmic reticulum stress
cellular response to vascular endothelial growth factor stimulus
peptidyl-serine autophosphorylation
IRE1-mediated unfolded protein response
protein autophosphorylation
mRNA splicing, via endonucleolytic cleavage and ligation
mRNA endonucleolytic cleavage involved in unfolded protein response
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to glucose stimulus
RNA phosphodiester bond hydrolysis, endonucleolytic
positive regulation of endoplasmic reticulum unfolded protein response
insulin metabolic process
peptidyl-serine trans-autophosphorylation
mRNA processing
rRNA catabolic process
apoptotic chromosome condensation
negative regulation of transcription, DNA-templated
RNA phosphodiester bond hydrolysis
apoptotic process
response to unfolded protein
metabolic process
phosphorylation
endoplasmic reticulum unfolded protein response
mRNA splicing via endonucleolytic cleavage and ligation involved in unfolded protein response
RNA splicing
nucleic acid phosphodiester bond hydrolysis
