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产品信息
PA1-46120
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Not Applicable
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide made the the N-terminal region of the human protein.
偶联物
形式
Liquid
浓度
2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1% BSA
内含物
no preservative
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2262247
产品详细信息
This ERO1L antibody is useful for Western Blot, where a band ~57 kDa is observed.
Depending on the samples used ERO1L can also appear as a doublet which is believed to represent the oxidized and reduced forms of the protein.
Suggested positive control: 293T, INS-1, and MIN6 cell lysate.
靶标信息
ERO1L (endoplasmic oxidoreductin-1-like protein) is localized in ER membrane as peripheral membrane protein on the lumenal side. Its association with ERP44 is essential for its retention in the ER and is unique in coupling oxygen reduction to de novo disulfides formation. The ERO1L enzyme is broadly distributed at low levels in several tissues with highest levels in the upper digestive tract and is stimulated by hypoxia via HIF-signaling. ERO1L generally exists as monomer, but has the ability to function as monomer or homodimer and interacts with PDILT. After translation, ERO1L gets n-glycosylated and the disulfide bonds constitute the redox-active center whereby the Cys-94/Cys-99 disulfide bond accepts an electron from P4HB followed by funneling the same to the active site disulfide Cys-394/Cys-397. ERO1L acts by oxidizing directly to P4HB/PDI isomerase through direct disulfide exchange and associates with ERP44. ERO1L is implicated in immunoglobulin folding, oxidative stress, release of unfolded cholera toxin from reduced P4HB/PDI upon Vibrio cholerae infection, and plays a key role in ER stress-induced CHOP-dependent apoptosis through inositol 1,4,5-trisphosphate receptor IP3R1 activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Endoplasmic oxidoreductin-1-like protein; endoplasmic reticulum oxidation 1; Endoplasmic reticulum oxidoreductase alpha; Endoplasmic reticulum oxidoreductin-1-like protein; ero1; ERO1-L; ERO1-L-alpha; ERO1-like protein alpha; Global ischemia-induced protein 11; Oxidoreductin-1-L-alpha
基因别名: ERO1-alpha; ERO1-L; ERO1-L-alpha; ERO1A; Ero1alpha; ERO1L; ERO1LA; Giig11; UNQ434/PRO865
UniProt ID: (Human) Q96HE7, (Mouse) Q8R180, (Rat) Q8R4A1
Entrez Gene ID: (Human) 30001, (Mouse) 50527, (Rat) 171562
protein disulfide isomerase activity
protein binding
protein disulfide oxidoreductase activity
disulfide oxidoreductase activity
oxidoreductase activity
oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor
peptide-methionine (S)-S-oxide reductase activity
peptide-methionine (R)-S-oxide reductase activity
methionine-R-sulfoxide reductase activity
oxidoreductase
response to reactive oxygen species
protein folding
cellular protein modification process
response to temperature stimulus
organ senescence
4-hydroxyproline metabolic process
protein maturation by protein folding
extracellular matrix organization
endoplasmic reticulum unfolded protein response
response to endoplasmic reticulum stress
cell redox homeostasis
brown fat cell differentiation
chaperone mediated protein folding requiring cofactor
release of sequestered calcium ion into cytosol
oxidation-reduction process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
cellular response to hypoxia
transport
apoptotic process
