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ERp72 Antibody (PA1-007) in ICC/IF
Immunofluorescent analysis of ERp72 (green) in 3T3 cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 15 minutes at room temperature. Cells were stained with a ERp72 rabbit polyclonal antibody (Product # PA1-007), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature, and then incubated with a Goat anti-rabbit IgG Superclonal secondary antibody, Alexa Fluor 488 conjugate (Product # A27034) at a dilution of 1:1000 ... View More
产品信息
PA1-007
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2160971
产品详细信息
PA1-007 detects ERp72 protein in human and mouse samples.
PA1-007 has successfully been used in ICC, IF and Western blot procedures. By Western blot, this antibody detects a 72 kDa protein representing ERp72 in human fibrosarcoma HT 1080 cell lysate.
The PA1-007 immunogen is a synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72. This peptide (Cat. # PEP-223) is available for use in neutralization and control experiments.
靶标信息
ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: calcium binding protein, intestinal; EC 5.3.4.1; endoplasmic reticulum protein 72; Endoplasmic reticulum resident protein 70; Endoplasmic reticulum resident protein 72; ER protein 70; ER protein 72; protein disu; protein disulfide isomerase family A, member 4; protein disulfide isomerase related protein (calcium-binding protein, intestinal-related); protein disulfide isomerase-associated 4; Protein disulfide-isomerase A4; Protein ERp-72; testicular tissue protein Li 137
基因别名: AI987846; Cai; ERp-72; ERP70; ERP72; PDIA4
UniProt ID: (Human) P13667, (Mouse) P08003
Entrez Gene ID: (Human) 9601, (Mouse) 12304
