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ERp72 Antibody (PA1-007-A647) in ICC/IF
Immunofluorescent analysis of ERp72 (red) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ERp72 Polyclonal Antibody, AlexaFluor 647 conjugate (Product # PA1-007-A647) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images... View More
产品信息
PA1-007-A647
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of humna ERp72
偶联物
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
保存条件
4° C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2662447
产品详细信息
PA1007A647 detects ERp72 protein in human and mouse samples.
The PA1007A647 immunogen is a synthetic peptide corresponding to residues K(629) F I E E H A T K L S R T K E E L(645) of human ERp72. This peptide (Cat. # PEP-223) is available for use in neutralization and control experiments.
靶标信息
ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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