Invitrogen

ESAM Monoclonal Antibody (1G8), PE, eBioscience™

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ESAM Antibody (12-5852-82) in Flow

Staining of bEnd.3 mouse endothelioma cell line with 0.125 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (open histogram) or 0.125 µg of Anti-Mouse ESAM PE (filled histogram). Total viable cells were used for analysis.

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产品信息

12-5852-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

查看4篇已发表文章 4篇已发表文章

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

1G8

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_891537

产品详细信息

Description: The 1G8 monoclonal antibody reacts with mouse ESAM (endothelial cell-selective adhesion molecule). ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.

Applications Reported: This 1G8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1G8 antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ESAM is a 55-kDa membrane protein composed of 2 extracellular Ig domains, a single transmembrane domain, and a 120 amino acid cytoplasmic domain. ESAM is related to members of the junctional adhesion molecule (JAM) family, and is expressed on endothelial cells and activated platelets. ESAM at endothelial tight junctions plays a role in the regulation of vascular permeability, in response to agents such as VEGF or extravasating neutrophils.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population.

Cell reports

Leylek R,Alcántara-Hernández M,Lanzar Z,Lüdtke A,Perez OA,Reizis B,Idoyaga J

Published figure using ESAM monoclonal antibody (Product # 12-5852-82) in Flow Cytometry

Tue Dec 10 00:00:00 UTC 2019

Expression of the Phosphatase Ppef2 Controls Survival and Function of CD8⁺ Dendritic Cells.

Frontiers in immunology

Zwick M,Ulas T,Cho YL,Ried C,Grosse L,Simon C,Bernhard C,Busch DH,Schultze JL,Buchholz VR,Stutte S,Brocker T

12-5852-82 was used in Flow Cytometry to suggest that Ppef2 is crucial to support survival of immature CD8+ DCs, while Ppef2 down-regulation during DC-maturation limits T cell responses.

Tue Oct 27 00:00:00 UTC 2020

Notch Signaling Facilitates In Vitro Generation of Cross-Presenting Classical Dendritic Cells.

Cell reports

Kirkling ME,Cytlak U,Lau CM,Lewis KL,Resteu A,Khodadadi-Jamayran A,Siebel CW,Salmon H,Merad M,Tsirigos A,Collin M,Bigley V,Reizis B

Tue Jun 19 00:00:00 UTC 2018

Notch2-dependent classical dendritic cells orchestrate intestinal immunity to attaching-and-effacing bacterial pathogens.

Nature immunology

Satpathy AT,Briseño CG,Lee JS,Ng D,Manieri NA,Kc W,Wu X,Thomas SR,Lee WL,Turkoz M,McDonald KG,Meredith MM,Song C,Guidos CJ,Newberry RD,Ouyang W,Murphy TL,Stappenbeck TS,Gommerman JL,Nussenzweig MC,Colonna M,Kopan R,Murphy KM

12-5852 was used in Flow cytometry/Cell sorting to elucidate the cellular source of essential IL-23 production required for the mucosal response to attaching-and-effacing bacterial infection.