Invitrogen

ESRP1 Polyclonal Antibody

ESRP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ESRP1 Antibody (PA5-25833) in IHC (P)

Immunohistochemistry analysis of ESRP1 in paraformaldehyde-fixed, paraffin-embedded human pancreas tissue sections. Samples were incubated with ESRP1 polyclonal antibody (Product # PA5-25833) using a dilution of 1:25 for 1 hours at 37°C followed by an undiluted biotinylated goat polyvalent antibody. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieva... View More

ESRP1 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

ESRP1 Antibody — Figure 5 ESRP1 expression influences CD44v6 expression and is epigenetically downregulated in advanced metastatic melanoma A. Scatter plots showing the correlation analysis of ESRP1 expression and CD44v6/s ratio using TCGA data for PRM. B. Box plot comparing the ESRP1 expression using TCGA RNA-Seq data for PRM, LNM, and DOM specimens. C. Box plots comparing the expression of ESRP1 assessed by qPCR for PRM, LNM, non-MBM, and MBM. D. Box plot comparing ESRP1 expression for PRM with and without MBM-development. E. Western blot showing ESRP1 and CD44v6 expression on two LNM cell lines (MB and LH) treated with siRNA control (siCTRL) and siRNA ESRP1 (siESRP1). F. Box plot comparing the DNA methylation level of ESRP1 gene in PRM, LNM, non-MBM, and MBM specimens. * is p = 0.05 and ** is p = 0.01. {KD}

产品信息

PA5-25833

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:50-1:100

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 182-211 amino acids of human ESRP1

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2543333

产品详细信息

This antibody is predicted to react with mouse and rat based on sequence homology.

靶标信息

RBM35A, also known as ESRP1, is a mRNA splicing factor that with its related protein RBM35B (ESRP2) are coordinators of an epithelial cell-type-specific splicing program. RBM35A contains three putative RNA recognition motifs and acts by directly binding specific sequences in mRNAs. RBM35A is involved in posttranscriptional regulation of a number of genes such as FGFR2, CD44, CTNND1, and ENAH by exerting a differential effect on protein translation via 5' UTRs of mRNAs. Other recent studies have shown that RMB35A may also act as a novel tumor suppressor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

CD44 alternative splicing senses intragenic DNA methylation in tumors via direct and indirect mechanisms.

Nucleic acids research

Batsché E,Yi J,Mauger O,Kornobis E,Hopkins B,Hanmer-Lloyd C,Muchardt C

PA5-25833 was used in Western Blot to explore the possibility of the impact of DNA methylation on alternative splicing that favors tumor progression.

Mon Jun 21 00:00:00 UTC 2021

Protocol for proteogenomic dissection of intronic splicing enhancer interactome for prediction of individualized cancer prognosis.

STAR protocols

Wang L,Wrobel JA,Xie L,Chen X

PA5-25833 was used in Western Blot to demonstrate a protocol for an alternative splicing activity-based proteogenomic approach for identification of candidate prognostic markers in cancer cell lines and human breast cancer specimens.