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ESRRB Antibody (PA1-315) in ICC/IF
Immunofluorescent analysis of Estrogen Related Receptor beta (green) in NRK cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a Estrogen Related Receptor beta polyclonal antibody (Product # PA1-315) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Produ... View More
产品信息
PA1-315
产品规格
种属反应
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues D(376) Y E L S Q R H E E P R R(388) of mouse ERR2.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2100260
产品详细信息
PA1-315 detects ERR2 from mouse and rat kidney extracts.
PA1-315 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~48 kDa protein representing ERR2 from rat kidney extract.
The PA1-315 immunogen is a synthetic peptide corresponding to residues D(376) Y E L S Q R H E E P R R(388) of mouse ERR2. This peptide (Cat. # PEP-251) is available for use in neutralization and control experiments.
靶标信息
ESRRB (steroid hormone receptor ERR2) plays a role in maintenance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. Upon FGF signaling pathway activation ESRRB interacts with KDM1A by directly binding to enhancer sites of ELF5 and EOMES and activates their transcription leading to self-renewal of trophoblast stem cells. ESSRB also regulates expression of multiple rod-specific genes and is required for survival of this cell type. Defects in the gene can cause deafness, autosomal recessive, 35 (DFNB35).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ERR-beta; ERRbeta; estrogen receptor related 2; Estrogen receptor-like 2; Estrogen-related receptor beta; estrogen-related receptor, beta; Nuclear receptor subfamily 3 group B member 2; Steroid hormone receptor ERR2
基因别名: Err-2; Err2; Errb; Esrl2; Esrrb; Estrrb; Nr3b2
UniProt ID: (Mouse) Q61539, (Rat) P11475
Entrez Gene ID: (Mouse) 26380, (Rat) 299210
RNA polymerase II distal enhancer sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
transcription coactivator activity
steroid binding
protein binding
transcription factor binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
in utero embryonic development
trophectodermal cellular morphogenesis
trophectodermal cell proliferation
embryonic placenta development
transcription, DNA-templated
regulation of transcription, DNA-templated
stem cell population maintenance
steroid hormone mediated signaling pathway
positive regulation of transcription from RNA polymerase II promoter
