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ETFB Antibody (PA5-106327) in ICC/IF
Immunofluorescent analysis of ETFB in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and ETFB polyclonal antibody (Product # PA5-106327) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-106327
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ETFB(Accession P38117), corresponding to amino acid residues A105-L155.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854004
Product Specific Information
Antibody detects endogenous levels of total ETFB.
Target Information
This gene encodes electron-transfer-flavoprotein, beta polypeptide, which shuttles electrons between primary flavoprotein dehydrogenases involved in mitochondrial fatty acid and amino acid catabolism and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. The gene deficiencies have been implicated in type II glutaricaciduria. Alternatively spliced transcript variants have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-ETF; electron transfer flavoprotein beta-subunit; Electron transfer flavoprotein subunit beta; electron transfer flavoprotein, beta polypeptide; electron transferring flavoprotein, beta polypeptide; electron-transfer-flavoprotein, beta polypeptide; electron-transferring-flavoprotein, beta polypeptide
Gene Aliases: 0610009I16Rik; 2810441H06Rik; ETFB; FP585; MADD
UniProt ID: (Human) P38117, (Rat) Q68FU3, (Mouse) Q9DCW4
Entrez Gene ID: (Human) 2109, (Rat) 292845, (Mouse) 110826
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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