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FIGURE: 1 / 1
ETFDH Antibody (PA5-109695) in WB
Western blot analysis of ETFDH in extracts of various cell lines. Samples were incubated with ETFDH Polyclonal antibody (Product # PA5-109695) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Product Details
PA5-109695
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 318-617 of human ETFDH (NP_004444.2).
Conjugate
Form
Liquid
Concentration
0.92 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855106
Product Specific Information
Immunogen sequence: LVALGLVVGL DYQNPYLSPF REFQRWKHHP SIRPTLEGGK RIAYGARALN EGGFQSIPKL TFPGGLLIGC SPGFMNVPKI KGTHTAMKSG ILAAESIFNQ LTSENLQSKT IGLHVTEYED NLKNSWVWKE LYSVRNIRPS CHGVLGVYGG MIYTGIFYWI LRGMEPWTLK HKGSDFERLK PAKDCTPIEY PKPDGQISFD LLSSVALSGT NHEHDQPAHL TLRDDSIPVN RNLSIYDGPE QRFCPAGVYE FVPVEQGDGF RLQINAQNCV HCKTCDIKDP SQNINWVVPE GGGGPAYNGM
Target Information
Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67-kDa precursor which is targeted to mitochondria and processed in a single step to a 64-kDa mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Electron transfer flavoprotein-ubiquinone oxidoreductase, mitochondrial; electron transferring flavoprotein dehydrogenase; Electron-transferring-flavoprotein dehydrogenase; ETF dehydrogenase; ETF-QO; ETF-ubiquinone oxidoreductase
Gene Aliases: 0610010I20Rik; AV001013; ETFDH; ETFQO; MADD
UniProt ID: (Human) Q16134, (Mouse) Q921G7
Entrez Gene ID: (Human) 2110, (Rat) 295143, (Mouse) 66841
electron-transferring-flavoprotein dehydrogenase activity
electron carrier activity
oxidoreductase activity
oxidoreductase activity, oxidizing metal ions with flavin as acceptor
metal ion binding
quinone binding
ubiquinone binding
flavin adenine dinucleotide binding
4 iron, 4 sulfur cluster binding
iron-sulfur cluster binding
oxidoreductase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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