Invitrogen

ETS1 Recombinant Rabbit Monoclonal Antibody (JM92-32)

ETS1 Antibody in Immunocytochemistry (ICC/IF)

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ETS1 Antibody (MA5-32732) in ICC/IF

Immunofluorescence analysis of ETS1 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ETS1 Monoclonal Antibody (Product # MA5-32732) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody (Product # A28177) at a dilution of 1:2000 f... View More

ETS1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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ETS1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. ETS1 was observed in Mouse Lung and Mouse Thymus but not in Mouse Cerebellum and Mouse Intestine using ETS1 Monoclonal Antibody (Product # MA5-32732) in western blot. {RE}

产品信息

MA5-32732

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

- -

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

5 µg/mL

流式细胞分析 (Flow)

1:50-1:100

免疫沉淀 (IP)

- -

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JM92-32

抗原

Synthetic peptide within Human ETS1 aa 10-59

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2810009

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

ETS1 (c-Ets-1; v-ets erythroblastosis virus E26 oncogene homlog 1 (avian); ETS-1; EWSR2; p54), a 441 A. A protein, is a member of DNA-binding ETS protein family. It is a multifunctional protein consisting of an N-terminal pointed domain, a central transactivation domain and 2 auto inhibitory domains flanked on both sides of conserved C-terminal Ets domain. It interacts with factors including PEBP2alphaA, p300/CBP and mediates transactivation of Osteopontin, Presenilin-1, Stromelysin and Collagenase. It is activated by Ras/MAPK signaling and its expression is up-regulated in resting T-cells. Its activity is modulated by various factors including AP-1, Sp-1, c-myb and MafB. Sp100 activates ETS1 in nuclear bodies. It interacts with EAP1/Daxx and causes repression of MMP1 and BCL2 transactivation. It interacts with STAT6 and modulates Socs-1 expression induced by IL-4. It interacts with GATA3 and positively regulates Tax-1 dependent IL-5 expression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1; avian erythroblastosis virus E26 oncogene 1; ets protein; Ets-1Delta(III-VI); FLJ10768; p54; Protein C-ets-1; tumor progression locus 1; v-ets avian erythroblastosis virus E2 oncogene homolog 1; v-ets avian erythroblastosis virus E26 oncogene homolog 1

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基因别名: AI196000; AI448617; c-ets-1; D230050P06; ETS-1; ETS1; EWSR2; p54; Tpl1

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UniProt ID: (Human) P14921, (Mouse) P27577

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Entrez Gene ID: (Human) 2113, (Mouse) 23871

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