Invitrogen

ETV4 Polyclonal Antibody

ETV4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 4

ETV4 Antibody (PA5-13595) in IHC (P)

Immunohistochemistry analysis of ETV4 in formalin-fixed and paraffin-embedded human brain tissue. Samples were incubated with ETV4 polyclonal antibody (Product # PA5-13595) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Product Image Gallery

产品信息

PA5-13595

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化（石蜡） (IHC (P))

1:10-1:50

流式细胞分析 (Flow)

1:10-1:50

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 419-447 amino acids from the C-terminal region of human ETV4

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Size-exclusion - Dialysis, Ammonium sulfate precipitation

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2246685

靶标信息

ETV4: ets variant gene 4 (E1A enhancer binding protein, E1AF), also known as PEA3. Several members of the Ets gene family are known to encode sequencespecific DNA binding proteins. These include mouse PU. 1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-a. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU. 1, although PU. 1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Adenovirus E1A enhancer-binding protein; E1A F; E1A-F; ETS translocation variant 4; ets variant gene 4 (E1A enhancer binding protein, E1AF); ets variant gene 4 (E1A enhancer-binding protein, E1AF); ETS variant protein 4; EWS protein/E1A enhancer binding protein chimera; PEA3 protein; Polyomavirus enhancer activator 3; POLYOMAVIRUS ENHANCER ACTIVATOR 3 (PEA3 PROTEIN) (ETS TRANSLOCATION VARIANT 4); Polyomavirus enhancer activator 3 homolog; Protein PEA3

查看更多收起

基因别名: AW414408; E1A-F; E1AF; ETV4; Pea-3; PEA3; PEAS3

查看更多收起

UniProt ID: (Human) P43268, (Mouse) P28322

查看更多收起

Entrez Gene ID: (Human) 2118, (Mouse) 18612

查看更多收起

功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding DNA binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding winged helix/forkhead transcription factor

参与通路