Invitrogen

EYA1 Polyclonal Antibody

查看其他6个EYA1抗体

抗体检测数据 (3)

EYA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EYA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

图: 1 / 3

EYA1 Antibody (PA5-117158) in IHC (P)

Immunohistochemistry analysis of EYA1 in paraffin-embedded human placenta. Samples were incubated with EYA1 Polyclonal antibody (Product # PA5-117158) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

EYA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EYA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EYA1 Antibody in Western Blot (WB)

Product Image Gallery

EYA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of EYA1 in paraffin-embedded human placenta. Samples were incubated with EYA1 Polyclonal antibody (Product # PA5-117158) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

EYA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of EYA1 in paraffin-embedded rat brain. Samples were incubated with EYA1 Polyclonal antibody (Product # PA5-117158) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

EYA1 Antibody in Western Blot (WB) — Western blot analysis of EYA1 in extracts of various cell lines. Samples were incubated with EYA1 Polyclonal antibody (Product # PA5-117158) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

产品信息

PA5-117158

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human EYA1 (NP_0004942)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.85 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2901788

产品详细信息

Positive Samples: SH-SY5Y, 293T, Mouse testis, Mouse brain, Rat brain

Immunogen sequence: NSLTNSSGFN SSQQDYPSYP SFGQGQYAQY YNSSPYPAHY MTSSNTSPTT PSTNATYQLQ EPPSGITSQA VTDPTAEYST IHSPSTPIKD SDSDRLRRGS D

靶标信息

This gene encodes a member of the eyes absent family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Four transcript variants encoding three distinct isoforms have been identified for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: EYA transcriptional coactivator and phosphatase 1; Eyes absent; eyes absent 1 homolog; Eyes absent homolog 1; MGC141875; OTTHUMP00000195053

查看更多收起

基因别名: BOP; BOR; BOS1; EYA1; OFC1

查看更多收起

UniProt ID: (Human) Q99502, (Mouse) P97767

查看更多收起

Entrez Gene ID: (Human) 2138, (Mouse) 14048, (Rat) 502935

查看更多收起

RNA binding protein tyrosine phosphatase activity protein binding metal ion binding phosphoprotein phosphatase activity hydrolase activity molecular_function

establishment of mitotic spindle orientation metanephros development branching involved in ureteric bud morphogenesis outflow tract morphogenesis double-strand break repair transcription, DNA-templated pattern specification process mesodermal cell fate specification sensory perception of sound anatomical structure morphogenesis response to ionizing radiation striated muscle tissue development histone dephosphorylation protein sumoylation cellular protein localization establishment or maintenance of apical/basal cell polarity peptidyl-tyrosine dephosphorylation aorta morphogenesis outer ear morphogenesis middle ear morphogenesis regulation of neuron differentiation positive regulation of DNA repair positive regulation of Notch signaling pathway positive regulation of transcription from RNA polymerase II promoter neuron fate specification embryonic skeletal system morphogenesis semicircular canal morphogenesis positive regulation of epithelial cell proliferation pharyngeal system development lung epithelial cell differentiation otic vesicle morphogenesis positive regulation of secondary heart field cardioblast proliferation cochlea morphogenesis negative regulation of extrinsic apoptotic signaling pathway in absence of ligand ureteric bud development DNA repair regulation of transcription, DNA-templated protein dephosphorylation cellular response to DNA damage stimulus multicellular organism development organ morphogenesis chromatin modification ear morphogenesis inner ear morphogenesis cell fate commitment positive regulation of transcription, DNA-templated anatomical structure development otic vesicle development biological_process