Invitrogen

EZH1 Polyclonal Antibody

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EZH1 Antibody (PA1-41114) in WB

Western blot was performed using Anti-EZH1 Polyclonal Antibody (Product # PA1-41114) and a ~90 kDa band corresponding to Histone-lysine N-methyltransferase EZH1 was observed along with few uncharacterised band (*) in the range of 80 kDa to 20 kDa across cell lines tested. Nuclear enriched extracts (30 µg lysate) of MOLT-4 (Lane 1), HCT 116 (Lane 2), TNFa treated HCT 116 (Lane 3), THP-1 (Lane 4), HEL 92.1.7 (Lane 5) and K-562 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were... View More

Product Image Gallery

EZH1 Antibody — Figure 7 Pharmacologic inhibition of EZH2 in SCLC inhibits growth in vitro and in vivo . ( a ) EZH1 and EZH2 are consistently expressed in a panel of SCLC PDX as measured by western blot ( b ) Ex vivo growth of the SCLC PDX LX92 is significantly inhibited by the EZH2 inhibitors EPZ-5687, GSK343 and UNC1999 as measured by resazurin conversion (two-way analysis of variance, adjusted for multiple comparisons by the method of Dunnet) ( c ) Western blot analysis of day 7 lysates from LX92 tumors treated with various EZH2 inhibitors. ( d ) In vivo growth of the SCLC PDX LX92 is significantly inhibited by the EZH2 inhibitor EPZ-6438. ( e ) Western blot analysis of end point LX92 tumor lysates treated with various EPZ-6438 treatment schedules. Tumors with growth inhibition greater than the median (TGI > median) for each treatment group were pooled separately from those with less robust response (TGI < median) to assess correlation between TGI and pharmacodynamic response. TGI, tumor growth inhibition. {TM}

产品信息

PA1-41114

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptides corresponding to a portion of amino acids 350-400 ofhuman EZH1.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2102295

产品详细信息

This antibody is 86% homologous to rat and canine.

Suggested positive control: antigen standard for EZH1 (transient overexpression lysate), human or mouse spleen.

靶标信息

EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 ).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

EED-Targeted PROTACs Degrade EED, EZH2, and SUZ12 in the PRC2 Complex.

Cell chemical biology

Hsu JH,Rasmusson T,Robinson J,Pachl F,Read J,Kawatkar S,O' Donovan DH,Bagal S,Code E,Rawlins P,Argyrou A,Tomlinson R,Gao N,Zhu X,Chiarparin E,Jacques K,Shen M,Woods H,Bednarski E,Wilson DM,Drew L,Castaldi MP,Fawell S,Bloecher A

PA1-41114 was used in Western Blotting to design, develop, and characterize novel EED-targeted PROTACs that can potently and selectively degrade both EED and associated proteins within the PRC2 complex.

Thu Jan 16 00:00:00 UTC 2020

DNA methylation in small cell lung cancer defines distinct disease subtypes and correlates with high expression of EZH2.

Oncogene

Poirier JT,Gardner EE,Connis N,Moreira AL,de Stanchina E,Hann CL,Rudin CM

PA1-41114 was used in western blot to investigate DNA methylation profiles in small cell lung cancer, patient-derived xenografts, and cell lines at single-nucleotide resolution