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EZH2 Antibody (702492) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MDA-MB-231 (Lane 1), MCF-7 (Lane 2), LNCaP (Lane 3), PC-3 (Lane 4), DU 145 (Lane 5), HCT 116 (Lane 6), SW480 (Lane 7), Caco-2 (Lane 8) and T98G (Lane 9). The blots were probed with Anti-EZH2 Recombinant Rabbit Monoclonal Antibody (Product # 702492, 0.5 µg/mL). A 85 kDa band corresponding to EZH2 was observed across the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conju... View More
产品信息
702492
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
8H32L43
抗原
Protein corresponding to human EZH2 (aa156-aa265)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2689469
产品详细信息
This antibody is predicted to react with Monkey, Rat, Horse, Mouse
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
EZH2 (Histone-lysine N-methyltransferase) is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Mutations affecting EZH2 can cause Weaver syndrome (WVS).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: an; Enhancer of zeste homolog 2; ENX 1; ENX-1; EZH 2; EZH-2; Histone-lysine N-methyltransferase EZH2; Lysine N-methyltransferase 6; OTTHUMP00000213774; OTTHUMP00000213778; OTTHUMP00000213779; OTTHUMP00000213780
基因别名: ENX-1; ENX1; EZH1; EZH2; EZH2b; KMT6; KMT6A; WVS; WVS2
UniProt ID: (Human) Q15910
Entrez Gene ID: (Human) 2146
core promoter binding
DNA binding
chromatin binding
RNA binding
protein binding
protein-lysine N-methyltransferase activity
histone-lysine N-methyltransferase activity
chromatin DNA binding
histone methyltransferase activity
ribonucleoprotein complex binding
sequence-specific DNA binding
histone methyltransferase activity (H3-K27 specific)
promoter-specific chromatin binding
histone modifying enzyme
negative regulation of transcription from RNA polymerase II promoter
DNA methylation
chromatin organization
transcription, DNA-templated
regulation of transcription, DNA-templated
positive regulation of epithelial to mesenchymal transition
regulation of gliogenesis
skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration
cerebellar cortex development
hippocampus development
response to estradiol
negative regulation of transcription elongation from RNA polymerase II promoter
regulation of cell proliferation
regulation of circadian rhythm
positive regulation of MAP kinase activity
negative regulation of sequence-specific DNA binding transcription factor activity
positive regulation of GTPase activity
negative regulation of epidermal cell differentiation
negative regulation of gene expression, epigenetic
negative regulation of transcription, DNA-templated
negative regulation of retinoic acid receptor signaling pathway
rhythmic process
negative regulation of striated muscle cell differentiation
cellular response to hydrogen peroxide
G1 to G0 transition
histone H3-K27 methylation
protein localization to chromatin
positive regulation of protein serine/threonine kinase activity
positive regulation of dendrite development
negative regulation of G1/S transition of mitotic cell cycle
