Invitrogen

Embigin Polyclonal Antibody

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Embigin Antibody (PA5-87887) in WB

Western blot analysis of Embigin in extracts of various cell lines. Samples were incubated with Embigin Polyclonal antibody (Product # PA5-87887) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

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产品信息

PA5-87887

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 33-260 of human EMB (NP_9408511)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.13 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2804481

产品详细信息

Immunogen sequence: DGSAPDSPFT SPPLREEIMA NNFSLESHNI SLTEHSSMPV EKNITLERPS NVNLTCQFTT SGDLNAVNVT WKKDGEQLEN NYLVSATGST LYTQYRFTII NSKQMGSYSC FFREEKEQRG TFNFKVPELH GKNKPLISYV GDSTVLTCKC QNCFPLNWTW YSSNGSVKVP VGVQMNKYVI NGTYANETKL KITQLLEEDG ESYWCRALFQ LGESEEHIEL VVLSYLVP; Positive Samples: Jurkat, MCF-7, THP-1; Cellular Location: Cell junction, Cell membrane, Single-pass type I membrane protein, synapse

靶标信息

Embigin is a 327 amino acid regulatory protein belonging to the immunoglobulin superfamily class of CAMs, and has two Ig-like (immunoglobulin-like) V- type domains. In situ hybridization analysis shows that Embigin has specific carbohydrate epitopes and plays an important role in early postimplantation embryogenesis. A highly glycosylated transmembrane glycoprotein, Embigin acts as a regulator of cell/ECM interactions during development and homeostasis of normal adult tissues. Transfection studies suggest its role in promotion of integrin-mediated cell-substratum adhesion. Widely expressed in most of the tissues and embryonal carcinoma cells, prostate and mammary cancer cell lines, it may also help in the classification of human carcinomas.

仅用于科研。不用于诊断过程。未经明确授权不得转售。