Invitrogen

Emerin Polyclonal Antibody

Emerin Antibody in Immunocytochemistry (ICC/IF)

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Emerin Antibody (PA5-29731) in ICC/IF

Immunofluorescence analysis of Emerin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Emerin Polyclonal Antibody (Product # PA5-29731) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790... View More

Emerin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

PA5-29731

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:10,000

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 48 and 254 of Human Emerin

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.01% thimerosal

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2547205

产品详细信息

Recommended positive controls: HeLa nucleus.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Xeroderma pigmentosum type G (XPG) is a human genetic disease exhibiting extreme sensitivity to sunlight. The XPG protein, a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family, is an enzyme essential for DNA repair of the major kinds of solar ultraviolet (UV)-induced DNA damages. Human XPG nuclease makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29-amino acid region of human XPG (residues 981-1009) contains the PCNA binding activity. A conserved arginine in XPG (Arg992) is crucial for its PCNA binding activity. Replication Protein A (RPA) binds specifically and directly to two excision repair proteins, the xeroderma pigmentosum damage-recognition protein XPA and the endonuclease XPG.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Combined loss of LAP1B and LAP1C results in an early onset multisystemic nuclear envelopathy.

Nature communications

Fichtman B,Zagairy F,Biran N,Barsheshet Y,Chervinsky E,Ben Neriah Z,Shaag A,Assa M,Elpeleg O,Harel A,Spiegel R

PA5-29731 was used in Western Blotting to determine the role of LAP1 isoforms, LAP1B and LAP1C, in early development and organogenesis.

Tue Feb 05 00:00:00 UTC 2019

Combined loss of LAP1B and LAP1C results in an early onset multisystemic nuclear envelopathy.

Nature communications

Fichtman B,Zagairy F,Biran N,Barsheshet Y,Chervinsky E,Ben Neriah Z,Shaag A,Assa M,Elpeleg O,Harel A,Spiegel R

PA5-29731 was used in Western Blotting to determine the role of LAP1 isoforms, LAP1B and LAP1C, in early development and organogenesis.

Tue Feb 05 00:00:00 UTC 2019

Topokaryotyping demonstrates single cell variability and stress dependent variations in nuclear envelope associated domains.

Nucleic acids research

Jurisic A,Robin C,Tarlykov P,Siggens L,Schoell B,Jauch A,Ekwall K,Sørensen CS,Lipinski M,Shoaib M,Ogryzko V

PA5-29731 was used in Immunocytochemistry-immunoflourescence to propose topokaryotyping as a technique to quantitatively analyse spatial position of genomic regions interacting with a nuclear landmark at the single cell level.