Invitrogen

Endocan Polyclonal Antibody

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Endocan Antibody (PA5-47206) in WB

Western blot analysis of Endocan in HUVEC human umbilical vein endothelial cells. Samples were incubated in Endocan polyclonal antibody (Product # PA5-47206) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Endocan/ESM‚1 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions.

Endocan Antibody in Neutralization (Neu)

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产品信息

PA5-47206

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

中和实验 (Neu)

1-4 µg/mL

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant human Endocan/ESM-1 Trp20-Arg184

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2577091

产品详细信息

In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant mouse Endocan is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

靶标信息

Endothelial cell-specific molecule 1 is a protein that in humans is encoded by the ESM1 gene. This gene encodes a secreted protein which is mainly expressed in the endothelial cells in human lung and kidney tissues. The expression of this gene is regulated by cytokines, suggesting that it may play a role in endothelium-dependent pathological disorders. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。