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Endostatin Antibody (500-P262-1MG) in WB
Western Blot: To detect Human Endostatin by Western Blot analysis Endostatin Polyclonal Antibody (Product # 500-P262-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Endostatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P262-1MG
产品规格
种属反应
Human
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Human Endostatin
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930140
产品详细信息
AA Sequence of recombinant protein: MHSHRDFQPV LHLVALNSPL SGGMRGIRGA DFQCFQQARA VGLAGTFRAF LSSRLQDLYS IVRRADRAAV PIVNLKDELL FPSWEALFSG SEGPLKPGAR IFSFDGKDVL RHPTWPQKSV WHGSDPNGRR LTESYCETWR TEAPSATGQA SSLLGGRLLG QSAASCHHAY IVLCIENSFM TASK.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Endostatin. Anti-Human Endostatin-specific antibody was purified by affinity chromatography employing an immobilized Human Endostatin matrix.
Sandwich ELISA: To detect Human Endostatin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Endostatin (500-P262Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Endostatin.
Western Blot: To detect Human Endostatin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Endostatin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
Endostatin is able to avoke non-uniform response for proliferation, cell mount and migration of entothelial cells, which different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce aquired drug resistance and has therefor become a potent new therapy strategy in solid neoplasias. This therapy appears to have a high potential not only for the treatment of gliomas, the most common brain tumors, but also in other tumors. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn^+2 binding and elastase processing. Widespread endostatin expression was found in elactic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: antiangiogenic agent; Collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; endostatin; FLJ27325; FLJ34914; MGC74745; multi-functional protein MFP
基因别名: COL18A1; KNO; KNO1; KS
UniProt ID: (Human) P39060
Entrez Gene ID: (Human) 80781
angiogenesis
endothelial cell morphogenesis
cell adhesion
visual perception
positive regulation of cell proliferation
negative regulation of cell proliferation
organ morphogenesis
extracellular matrix organization
positive regulation of cell migration
collagen catabolic process
response to drug
response to hydrostatic pressure
positive regulation of endothelial cell apoptotic process
