Invitrogen

Endostatin Polyclonal Antibody

Endostatin Antibody in Immunocytochemistry (ICC/IF)

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Endostatin Antibody (PA1-601) in ICC/IF

Immunofluorescent analysis of Endostatin (green) showing positive staining in the secretion of NIH-3T3 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature and probed with an Endostatin polyclonal antibody (Product # PA1-601) ... View More

Endostatin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Endostatin Antibody in Western Blot (WB)

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产品信息

PA1-601

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:200-1:1,000

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Non-human primate

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptides corresponding to residues R(1718) R L M E S Y C E T W R T E(1731) of Mouse Endostatin and R(1699) R L T E S Y C E T W R T E(1712) of Human Endostatin

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2245014

产品详细信息

PA1-601 detects recombinant human and mouse endostatin.

PA1-601 has been successfully used in Western blot, IF/ICC and IHC (P) procedures. By Western blot, this antibody detects a 20 kDa protein representing recombinant human endostatin.

PA1-601 immunizing peptides are two peptides derived from mouse and human endostatin protein corresponding to amino acid residues 129-142 of endostatin.

PA1-601 can be used with blocking peptide PEP-112.

靶标信息

Endostatin is able to avoke non-uniform response for proliferation, cell mount and migration of entothelial cells, which different endostatin binding characteristic, leads to the assumption that endostatin effect is strongly dependent from endothelial cell type. Furthermore endostatin inhibits angiogenesis and tumor growth in vivo by inducing apoptosis in endothelial cells. The local delivery of endostatin seems to specifically affect tumor-associated microvessels by reduction of the vessel density, diameter and functionality. Tumor cell migration and invasion was greatly reduced in the endostatin treated animals. Endostatin is non-toxic and does not induce aquired drug resistance and has therefor become a potent new therapy strategy in solid neoplasias. This therapy appears to have a high potential not only for the treatment of gliomas, the most common brain tumors, but also in other tumors. The ability of endostatin to inhibit neoangiogenesis is mediated, at least in part, by Zn^+2 binding and elastase processing. Widespread endostatin expression was found in elactic fibers in vessel walls and in some other basement membrane zones. Endostatin is released by neurons to accumulate in amyloid plaques in Alzheimer's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization.

Experimental eye research

Bonet A,Valença A,Mendes-Jorge L,Casellas A,Rodríguez-Baeza A,Nacher V,Ramos D,Pampalona J,Simó R,Ruberte J

PA1-601 was used in Immunohistochemistry to analyse the intracellular distribution of endostatin in healthy mouse and human neuroretinas.

Mon Nov 01 00:00:00 UTC 2021

Decreased endostatin in db/db retinas is associated with optic disc intravitreal vascularization.

Experimental eye research

Bonet A,Valença A,Mendes-Jorge L,Casellas A,Rodríguez-Baeza A,Nacher V,Ramos D,Pampalona J,Simó R,Ruberte J

PA1-601 was used in Immunohistochemistry to analyse the intracellular distribution of endostatin in healthy mouse and human neuroretinas.

Mon Nov 01 00:00:00 UTC 2021

Interaction between prostate cancer cells and prostate fibroblasts promotes accumulation and proteolytic processing of basement membrane proteins.

The Prostate

Ojalill M,Virtanen N,Rappu P,Siljamäki E,Taimen P,Heino J

PA1-601 was used in Western Blotting to unveil the matrisome in 3D spheroids formed by DU145 prostate cancer cells, PC3 prostate cancer cells, or prostate-derived fibroblasts.

Mon Jun 01 00:00:00 UTC 2020

Fibronectin Regulation of Integrin B1 and SLUG in Circulating Tumor Cells.

Cells

Huaman J,Naidoo M,Zang X,Ogunwobi OO

PA1-601 was used in Immunohistochemistry-immunofluorescence to show CTCs demonstrate enhanced migration in part through fibronectin regulation of integrin B1 and SLUG.

Thu Jun 20 00:00:00 UTC 2019

C3G promotes a selective release of angiogenic factors from activated mouse platelets to regulate angiogenesis and tumor metastasis.

Oncotarget

Martín-Granado V,Ortiz-Rivero S,Carmona R,Gutiérrez-Herrero S,Barrera M,San-Segundo L,Sequera C,Perdiguero P,Lozano F,Martín-Herrero F,González-Porras JR,Muñoz-Chápuli R,Porras A,Guerrero C

Published figure using Endostatin polyclonal antibody (Product # PA1-601) in Immunofluorescence

Tue Dec 19 00:00:00 UTC 2017

Gene transfer of endostatin enhances the efficacy of doxorubicin to suppress human hepatocellular carcinomas in mice.

Cancer science

Liu F,Tan G,Li J,Dong X,Krissansen GW,Sun X

PA1-601 was used in western blot to investigate the effect of gene transfer of endostatin on the therapeutic efficacy of doxorubicin against human hepatocellular carcinomas

Sat Sep 01 00:00:00 UTC 2007

生物信息学

蛋白别名: antiangiogenic agent; Collagen alpha-1(XVIII) chain; collagen, type XVIII, alpha 1; Endostatin; FLJ27325; FLJ34914; MGC74745; multi-functional protein MFP; NC1; Non-collagenous domain 1; procollagen, type XVIII, alpha 1

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基因别名: COL18A1; KNO; KNO1; KS

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UniProt ID: (Human) P39060, (Mouse) P39061

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Entrez Gene ID: (Human) 80781, (Mouse) 12822

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功能

structural molecule activity metal ion binding identical protein binding

参与通路