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EpCAM (CD326) Antibody (PA5-119097) in IHC (P)
Immunohistochemistry analysis of EpCAM (CD326) in paraffin-embedded Porcine Small intestine tissue. Samples were incubated with polyclonal antibody (Product # PA5-119097) at a dilution of 10 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 2 µg/mL.
Product Details
PA5-119097
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Pig
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein Epithelial Cell Adhesion Molecule. The antigen corresponds to amino acid range 25-265 of the target protein.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2903597
Target Information
Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule. Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E. Ep-CAM mediates Ca2+-independent homotypic interactions. Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells. Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation. Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage. Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types. Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD326; Ep-CAM; Epithelial cell adhesion molecule; epithelial glycoprotein; hEGP314; Tumor-associated calcium signal transducer 1
Gene Aliases: EGP; Ep-CAM; EPCAM; TACSTD1
UniProt ID: (Pig) Q75QW1
Entrez Gene ID: (Pig) 403163
ureteric bud development
positive regulation of cell proliferation
signal transduction involved in regulation of gene expression
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
cell-cell adhesion
negative regulation of cell-cell adhesion mediated by cadherin
positive regulation of stem cell proliferation
Performance Guarantee
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