Invitrogen

EphA2 Polyclonal Antibody

EphA2 Antibody in Immunocytochemistry (ICC/IF)

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EphA2 Antibody (34-7400) in ICC/IF

Immunofluorescence analysis of EphA2 Receptor was performed using 90% confluent log phase A549 cells treated with UV (302 nm) for 60 minutes without recovery. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EphA2 Receptor Rabbit Polyclonal Antibody (Product # 34-7400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain... View More

EphA2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

EphA2 Antibody in Immunohistochemistry (IHC)

EphA2 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

EphA2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence of EPHA2 receptor using Anti - EPHA2 receptor antibody (Product # 34-7400), shows increased expression of Anti-EPHA2 receptors in in A549 cell lines upon UV treatment. {TM}

产品信息

34-7400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看8篇已发表文章 8篇已发表文章

免疫组化 (IHC)

查看8篇已发表文章 8篇已发表文章

免疫组化（石蜡） (IHC (P))

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

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流式细胞分析 (Flow)

查看3篇已发表文章 3篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

查看2篇已发表文章 2篇已发表文章

肽阵列 (Array)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the C-terminal region of the human EphA2 receptor protein.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533185

靶标信息

EPHA2 belongs to the ephrin receptor subfamily of the protein tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediated developmental events, particularly in the nervous system. EPHA2 is overexpressed in many types of human cancers. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease.

JBMR plus

Vaught DB,Merkel AR,Lynch CC,Edwards J,Tantawy MN,Hilliard T,Wang S,Peterson T,Johnson RW,Sterling JA,Brantley-Sieders D

34-7400 was used in ELISA,Western Blot,Immunohistochemistry to provide a strong rationale for the development and application of molecularly targeted therapies against EphA2 for the treatment of breast cancer bone metastatic disease.

Thu Apr 01 00:00:00 UTC 2021

EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease.

JBMR plus

Vaught DB,Merkel AR,Lynch CC,Edwards J,Tantawy MN,Hilliard T,Wang S,Peterson T,Johnson RW,Sterling JA,Brantley-Sieders D

Thu Apr 01 00:00:00 UTC 2021

EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease.

JBMR plus

Vaught DB,Merkel AR,Lynch CC,Edwards J,Tantawy MN,Hilliard T,Wang S,Peterson T,Johnson RW,Sterling JA,Brantley-Sieders D

Thu Apr 01 00:00:00 UTC 2021

EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease.

JBMR plus

Vaught DB,Merkel AR,Lynch CC,Edwards J,Tantawy MN,Hilliard T,Wang S,Peterson T,Johnson RW,Sterling JA,Brantley-Sieders D

Thu Apr 01 00:00:00 UTC 2021

EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease.

JBMR plus

Vaught DB,Merkel AR,Lynch CC,Edwards J,Tantawy MN,Hilliard T,Wang S,Peterson T,Johnson RW,Sterling JA,Brantley-Sieders D

Thu Apr 01 00:00:00 UTC 2021

EphA2 Is a Clinically Relevant Target for Breast Cancer Bone Metastatic Disease.

JBMR plus

Vaught DB,Merkel AR,Lynch CC,Edwards J,Tantawy MN,Hilliard T,Wang S,Peterson T,Johnson RW,Sterling JA,Brantley-Sieders D

Thu Apr 01 00:00:00 UTC 2021

EphA2 as a new target for breast cancer and its potential clinical application.

International journal of clinical and experimental pathology

Zhou L,Lu X,Zhang B,Shi Y,Li Z

34-7400 was used in Immunocytochemistry to study EphA2 expression and suitability as a new breast cancer target.

Tue May 04 00:00:00 UTC 2021

Phosphorylation of PLCγ1 by EphA2 Receptor Tyrosine Kinase Promotes Tumor Growth in Lung Cancer.

Molecular cancer research : MCR

Song W,Kim LC,Han W,Hou Y,Edwards DN,Wang S,Blackwell TS,Cheng F,Brantley-Sieders DM,Chen J

34-7400 was used in Immunohistochemistry (Paraffin) to to show that the EphA2-PLCγ1 signaling axis promotes tumor growth of lung cancer and provides rationale for disruption of this signaling axis as a potential therapeutic option.

Sun Nov 01 00:00:00 UTC 2020

Cytotoxic effect of Ad-ephrinA1-caspase-3-T on breast cancer cells in vitro.

Translational cancer research

Huang Y,Zhang B,Li Y,Li Z,Zhang Q,Zhao B

34-7400 was used in Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry to demontrate that Ad-ephrinA1-caspase-3-T has a targeted cytotoxic effect on breast cancer cells in vitro.

Fri Feb 01 00:00:00 UTC 2019

The receptor tyrosine kinase EphA2 promotes glutamine metabolism in tumors by activating the transcriptional coactivators YAP and TAZ.

Science signaling

Edwards DN,Ngwa VM,Wang S,Shiuan E,Brantley-Sieders DM,Kim LC,Reynolds AB,Chen J

34-7400 was used in Immunohistochemistry to study the mechanism of EphA2-mediated glutaminolysis in human breast cancer.

Tue Dec 05 00:00:00 UTC 2017

Targeting EphA2 impairs cell cycle progression and growth of basal-like/triple-negative breast cancers.

Oncogene

Song W,Hwang Y,Youngblood VM,Cook RS,Balko JM,Chen J,Brantley-Sieders DM

Thu Oct 05 00:00:00 UTC 2017

Targeting EphA2 impairs cell cycle progression and growth of basal-like/triple-negative breast cancers.

Oncogene

Song W,Hwang Y,Youngblood VM,Cook RS,Balko JM,Chen J,Brantley-Sieders DM

Thu Oct 05 00:00:00 UTC 2017

Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery.

International journal of molecular sciences

Stammes MA,Prevoo HA,Ter Horst MC,Groot SA,Van de Velde CJ,Chan AB,de Geus-Oei LF,Kuppen PJ,Vahrmeijer AL,Pasquale EB,Sier CF

347400 was used in immunohistochemistry - paraffin section to examine if EphA2 and EphB4 proteins are suitable targets for image-guided oncologic surgery of colorectal cancer

Fri Feb 03 00:00:00 UTC 2017

Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery.

International journal of molecular sciences

Stammes MA,Prevoo HA,Ter Horst MC,Groot SA,Van de Velde CJ,Chan AB,de Geus-Oei LF,Kuppen PJ,Vahrmeijer AL,Pasquale EB,Sier CF

347400 was used in immunohistochemistry - paraffin section to examine if EphA2 and EphB4 proteins are suitable targets for image-guided oncologic surgery of colorectal cancer

Fri Feb 03 00:00:00 UTC 2017

Evaluation of EphA2 and EphB4 as Targets for Image-Guided Colorectal Cancer Surgery.

International journal of molecular sciences

Stammes MA,Prevoo HA,Ter Horst MC,Groot SA,Van de Velde CJ,Chan AB,de Geus-Oei LF,Kuppen PJ,Vahrmeijer AL,Pasquale EB,Sier CF

347400 was used in immunohistochemistry - paraffin section to examine if EphA2 and EphB4 proteins are suitable targets for image-guided oncologic surgery of colorectal cancer

Fri Feb 03 00:00:00 UTC 2017

EPHA2 Blockade Overcomes Acquired Resistance to EGFR Kinase Inhibitors in Lung Cancer.

Cancer research

Amato KR,Wang S,Tan L,Hastings AK,Song W,Lovly CM,Meador CB,Ye F,Lu P,Balko JM,Colvin DC,Cates JM,Pao W,Gray NS,Chen J

34-7400 was used in immunohistochemistry to report the role for EPHA2 in the cell survival of tyrosine kinase inhibitors-resistant, EGFR-mutant lung cancers

Fri Jan 15 00:00:00 UTC 2016

Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation.

Molecular biology of the cell

Barquilla A,Lamberto I,Noberini R,Heynen-Genel S,Brill LM,Pasquale EB

347400 was used in immunoprecipitation and western blot to screen for inhibitors of EphA2 canonical signaling

Thu Sep 01 00:00:00 UTC 2016

Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation.

Molecular biology of the cell

Barquilla A,Lamberto I,Noberini R,Heynen-Genel S,Brill LM,Pasquale EB

347400 was used in immunoprecipitation and western blot to screen for inhibitors of EphA2 canonical signaling

Thu Sep 01 00:00:00 UTC 2016

Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer.

Oncotarget

Efazat G,Novak M,Kaminskyy VO,De Petris L,Kanter L,Juntti T,Bergman P,Zhivotovsky B,Lewensohn R,Hååg P,Viktorsson K

347400 was used in immunohistochemistry - paraffin section, proximity ligation assay, and western blot to study the impact of ephrin signaling in non-small cell lung cancer

Tue Sep 13 00:00:00 UTC 2016

Ephrin B3 interacts with multiple EphA receptors and drives migration and invasion in non-small cell lung cancer.

Oncotarget

Efazat G,Novak M,Kaminskyy VO,De Petris L,Kanter L,Juntti T,Bergman P,Zhivotovsky B,Lewensohn R,Hååg P,Viktorsson K

347400 was used in immunohistochemistry - paraffin section, proximity ligation assay, and western blot to study the impact of ephrin signaling in non-small cell lung cancer

Tue Sep 13 00:00:00 UTC 2016

Improved efficacy of mitoxantrone in patients with castration-resistant prostate cancer after vaccination with GM-CSF-transduced allogeneic prostate cancer cells.

Oncoimmunology

van Dodewaard-de Jong JM,Santegoets SJ,van de Ven PM,Versluis J,Verheul HM,de Gruijl TD,Gerritsen WR,van den Eertwegh AJ

34-7400 was used in Flow cytometry/Cell sorting to evaluate the efficacy of chemotherapy in castration-resistant prostate cancer patients, following immunotherapy.

Fri Apr 01 00:00:00 UTC 2016

Genetic and pharmacologic inhibition of EPHA2 promotes apoptosis in NSCLC.

The Journal of clinical investigation

Amato KR,Wang S,Hastings AK,Youngblood VM,Santapuram PR,Chen H,Cates JM,Colvin DC,Ye F,Brantley-Sieders DM,Cook RS,Tan L,Gray NS,Chen J

34-7400 was used in immunohistochemistry to study the effect of EPHA2 inhibition on cell apoptosis in non-small cell lung cancers.

Thu May 01 00:00:00 UTC 2014

Evaluation of Eph receptor and ephrin expression within the human cornea and limbus.

Experimental eye research

Hogerheyde TA,Stephenson SA,Harkin DG,Bray LJ,Madden PW,Woolf MI,Richardson NA

Fri Feb 01 00:00:00 UTC 2013

Profiling Eph receptor expression in cells and tissues: a targeted mass spectrometry approach.

Cell adhesion & migration

Noberini R,Rubio de la Torre E,Pasquale EB

Published figure using EphA2 polyclonal antibody (Product # 34-7400) in Western Blot

Wed Nov 28 00:00:00 UTC 2012

Eph/ephrin profiling in human breast cancer reveals significant associations between expression level and clinical outcome.

PloS one

Brantley-Sieders DM,Jiang A,Sarma K,Badu-Nkansah A,Walter DL,Shyr Y,Chen J

34-7400 was used in western blot to study eph and ephrin expression in human breast cancer and correlate it with the overall and/or recurrence-free survival.

Tue Feb 28 00:00:00 UTC 2012

Eph/ephrin profiling in human breast cancer reveals significant associations between expression level and clinical outcome.

PloS one

Brantley-Sieders DM,Jiang A,Sarma K,Badu-Nkansah A,Walter DL,Shyr Y,Chen J

34-7400 was used in western blot to study eph and ephrin expression in human breast cancer and correlate it with the overall and/or recurrence-free survival.

Tue Feb 28 00:00:00 UTC 2012

Loss of EphA2 receptor tyrosine kinase reduces ApcMin/+ tumorigenesis.

International journal of cancer

Bogan C,Chen J,O'Sullivan MG,Cormier RT

Sun Mar 15 00:00:00 UTC 2009

The EphA4 receptor regulates neuronal morphology through SPAR-mediated inactivation of Rap GTPases.

The Journal of neuroscience : the official journal of the Society for Neuroscience