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Ephrin B1, B2, B3 Polyclonal Antibody

Ephrin B1, B2, B3 Antibody in Immunocytochemistry (ICC/IF)

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Ephrin B1, B2, B3 Antibody (600-401-MP0) in ICC/IF

Immunofluorescence of Rabbit Anti-Ephrin B1-B3 Antibody. (A). Neural Plate of Xenopus laevis stage 17. (B). Knockdown neural plate of Xenopus laevis stage 17. Primary Antibody: Anti-Ephrin B1-3 at 1:5000 overnight at 2-8°C. Secondary Antibody: Anti-Rabbit IgG Fluorescent Secondary. Fixative: 4% Paraformaldehyde in PBS. Permeabilization: 0.05% Triton-X100 in PBS. Nuclear Counterstain: None. Magnifica... View More

Ephrin B1, B2, B3 Antibody in Western Blot (WB)

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产品信息

600-401-MP0

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫细胞化学 (ICC/IF)

1:5,000

酶联免疫吸附实验 (ELISA)

1:10,000-1:50,000

产品规格

种属反应

Human, Mouse, Xenopus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Anti-Ephrin B1-B3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human Ephrin B1, B2, B3 conjugated to Keyhole Limpet Hemocyanin (KLH).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.05 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

内含物

0.01% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human Ephrin B1, B2, B3. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with Ephrin-B1 to human, mouse, rat, and Gallus gallus; 100% homology with Ephrin-B2 to mouse; 100% homology with Ephrin-B3 to human and mouse.

靶标信息

Eph receptors and ephrins exhibit complementary expression in many tissues during embryogenesis indicating that bidirectional activation of Eph receptors and ephrin-B proteins may occur at expression domain interfaces. Ephrin-B1 transduces outside-in signals through C-terminal protein interactions that effect integrin-mediated cell attachment and migration. The distribution of ephrin-B1 in the developing retina suggests that it influences retinal axon mapping along the dorsal-ventral axis and may be involved in intratectal development The transmembrane ligand ephrin-B2 and its receptor tyrosine kinase EphB4 are specifically expressed on arterial and venous endothelial cells, respectively.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CEK5 receptor ligand; CEK5-L; EFL-3; ELF-2; ELK ligand; ELK-L; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 2; EPH-related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 8; Ephrin-B1; Ephrin-B2; Ephrin-B3; HTK ligand; HTK-L; LERK-2; LERK-5; LERK-8; ligand of eph-related kinase 5; Stimulated by retinoic acid gene 1 protein

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基因别名: Cek5-L; CFND; CFNS; EFB1; EFL-3; EFL-6; EFL3; EFL6; EFNB1; EFNB2; EFNB3; ELF-2; ELF-3; Elf2; Elk-L; Elk-L3; Epl2; Epl5; Epl8; EPLG2; EPLG5; EPLG8; Htk-L; HTKL; LERK-2; LERK-5; LERK-8; LERK2; LERK5; LERK8; NLERK-1; NLERK-2; Stra1