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ErbB2 (HER-2) Antibody (BMS120) in ICC/IF
Immunofluorescence analysis of ErbB2 was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Her-2 Mouse monoclonal Antibody (Product # BMS120) at 5µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsi... View More
产品信息
BMS120
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2G11
抗原
Rec. Human HER-2
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2-7.4
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_10597305
产品详细信息
Description: The antibody (2G11) can be used to stain cryostat sections or cell smears. BMS120 is also suitable as primary antibody in staining for FACS analysis, BMS120FI can be used for direct staining of cells, BMS120BT can be used in a 2-step procedure using the biotin-(strept)avidin system.
The epitope is located in the extracellular domain, no cross reactivity with EGF-R has been detected by immunoprecipitation.
Both HER-2 oncogene amplification and oncoprotein overexpression have been correlated with breast, ovarian, gastric, lung, and other cancers.
In 15-40 % of primary breast cancers, amplification of the HER-2 oncogen is found which is highly correlated with overexpression of the encoded protein and seems to play a major role especially during the initiation of ductal carcinomas. HER-2 overexpression is described as independent prognostic factor with greater predictive power than most of the currently used prognostic tools - especially in axillary lymph-node-positive breast cancer patients.
32 % of ovarian carcinomas overexpress the HER-2 oncoprotein. Survival of those patients is significantly worse compared with cases of normal HER-2 protein expression. Additionally, patients whose tumors have high HER-2 protein expression are significantly less likely to have a complete response to primary therapy. A correlation between HER-2 expression, and clinical outcome has been also demonstrated for head and neck, salivary gland and placental carcinomas.
HER-2 is useful in identifying cancer cells with increased aggressiveness. Soluble p97-115 HER-2 (the soluble circulating fragment of p185 HER-2) protein levels in serum can be used as diagnostic tool for monitoring the extent of tumor spread, postoperative relapse and/or metastatic risk for different cancers.
Applications Tested: Immunohistochemistry (Frozen), FlowCytometry.
Purity: >95%.
靶标信息
ErbB2 (HER2) is a receptor tyrosine kinase that is overexpressed in some breast tumors. Herceptin, used in treatment of metastatic Her2-positive cancer, is monoclonal antibody targeting this kinase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: c-erb B2/neu protein; CD antigen CD340; CD340; erb-b2; erbb 2; ErbB2 (pTyr1139); ErbB2 (pY1139); ErbB2 phospho Y1139; her 2; herstatin; human epidermal growth factor receptor 2; metas; Metastatic lymph node gene 19 protein; MLN 19; neuro/glioblastoma derived oncogene homolog; neuroblastoma/glioblastoma derived oncogene homolog; p185erbB2; phospho ErbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; Receptor tyrosine-protein kinase erbB-2; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2; v-erb-b2 avian erythroblastic leukemia viral oncoprotein 2; v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
基因别名: CD340; ERBB2; HER-2; HER-2/neu; HER2; MLN 19; MLN19; NEU; NGL; TKR1
UniProt ID: (Human) P04626
Entrez Gene ID: (Human) 2064
RNA polymerase I core binding
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor signaling protein tyrosine kinase activity
transmembrane signaling receptor activity
Ras guanyl-nucleotide exchange factor activity
protein binding
ATP binding
protein C-terminus binding
growth factor binding
protein phosphatase binding
identical protein binding
ErbB-3 class receptor binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
protein heterodimerization activity
protein dimerization activity
transmembrane signal receptor
MAPK cascade
positive regulation of protein phosphorylation
transcription, DNA-templated
protein phosphorylation
signal transduction
cell surface receptor signaling pathway
enzyme linked receptor protein signaling pathway
transmembrane receptor protein tyrosine kinase signaling pathway
peripheral nervous system development
heart development
neuromuscular junction development
motor neuron axon guidance
cell proliferation
phosphatidylinositol 3-kinase signaling
regulation of phosphatidylinositol 3-kinase signaling
peptidyl-tyrosine phosphorylation
positive regulation of cell growth
regulation of microtubule-based process
negative regulation of immature T cell proliferation in thymus
ERBB2 signaling pathway
wound healing
myelination
positive regulation of MAP kinase activity
positive regulation of GTPase activity
positive regulation of translation
regulation of angiogenesis
positive regulation of cell adhesion
positive regulation of transcription from RNA polymerase I promoter
positive regulation of transcription from RNA polymerase III promoter
protein autophosphorylation
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
oligodendrocyte differentiation
positive regulation of epithelial cell proliferation
regulation of ERK1 and ERK2 cascade
cellular response to growth factor stimulus
cellular response to epidermal growth factor stimulus
positive regulation of protein targeting to membrane
regulation of cell motility
