Invitrogen

Estrogen Receptor alpha Polyclonal Antibody

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17 篇参考文献
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产品信息

PA1-308

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1 µg/mL
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免疫组化 (IHC)

-
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免疫组化（冰冻） (IHC (F))

1-5 µg/mL
-

免疫细胞化学 (ICC/IF)

1:1,000-1:4,000
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产品规格

种属反应

Human, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha.
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_325813

产品详细信息

PA1-308 has been successfully used in Western blot, immunofluorescence and immunohistochemistry procedures. By Western blot, this antibody detects an ~64 kDa protein representing endogenous levels of ER alpha from MCF-7 cell extracts. Immunohistochemical staining of ER alpha in rat prostate with PA1-308 results in primarily nuclear staining.

The PA1-308 immunogen is a synthetic peptide corresponding to residues M(1) T M T L H T K A S G M A L L H Q I Q G N(21) C of human ER alpha. This peptide sequence is completely conserved between human, rat and mouse and is highly conserved between sheep, porcine, camel, bovine and chicken ER alpha. PA1-308 immunizing peptide (Cat. # PEP-036) is available for use in neutralization and control experiments.

Recombinant ER alpha expressed in baculovirus system (Cat. # RP-310) can be purchased for control experiments in Western blot and gel shift assays.

靶标信息

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors. The estrogen receptor is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to estrogen response elements (ERE) in DNA. When bound to EREs, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Due to alternative RNA splicing, at least 4 estrogen receptor-alpha isoforms are known to exist (Isoform 1 (66 kDa), Isoform 2 (53 kDa), Isoform 3 (47 kDa), Isoform 4 (35 kDa)). Estrogen receptors are widely expressed in different tissue types and are essential for sexual development and reproductive function. They also play a role in other tissues such as bone. Estrogen receptors are involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: DKFZp686N23123; ER; Er alpha; ER-alpha; ER36; esr 1; esr-1; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor protein; hER-alpha36; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1

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基因别名: ER; ER-alpha; Era; ESR; ESR1; ESRA; Estr; ESTRR; NR3A1; RNESTROR

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UniProt ID: (Human) P03372, (Rat) P06211

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Entrez Gene ID: (Human) 2099, (Rat) 24890

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功能
RNA polymerase II core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription coactivator binding chromatin binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity steroid binding protein binding beta-catenin binding transcription factor binding zinc ion binding enzyme binding nitric-oxide synthase regulator activity estrogen receptor activity type 1 metabotropic glutamate receptor binding protein complex binding estrogen response element binding phosphatidylinositol 3-kinase regulatory subunit binding RNA polymerase II transcription factor activity, estrogen-activated sequence-specific DNA binding hormone binding identical protein binding ATPase binding DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
参与通路
negative regulation of transcription from RNA polymerase II promoter antral ovarian follicle growth osteoblast development chromatin remodeling transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter transcription initiation from RNA polymerase II promoter signal transduction phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration androgen metabolic process negative regulation of gene expression positive regulation of phospholipase C activity intracellular steroid hormone receptor signaling pathway intracellular estrogen receptor signaling pathway response to estradiol negative regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of sequence-specific DNA binding transcription factor activity regulation of neuron apoptotic process response to estrogen positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of glucose import decidualization positive regulation of fibroblast proliferation negative regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of sequence-specific DNA binding transcription factor activity Sertoli cell development Sertoli cell proliferation uterus development vagina development prostate epithelial cord elongation prostate epithelial cord arborization involved in prostate glandular acinus morphogenesis regulation of branching involved in prostate gland morphogenesis mammary gland branching involved in pregnancy mammary gland alveolus development epithelial cell proliferation involved in mammary gland duct elongation positive regulation of ERK1 and ERK2 cascade protein localization to chromatin cellular response to estrogen stimulus cellular response to estradiol stimulus negative regulation of triglyceride metabolic process negative regulation of neuron death negative regulation of production of miRNAs involved in gene silencing by miRNA baculum development epithelial cell development regulation of transcription from RNA polymerase II promoter male gonad development intracellular receptor signaling pathway regulation of apoptotic process steroid hormone mediated signaling pathway positive regulation of nitric oxide biosynthetic process positive regulation of retinoic acid receptor signaling pathway positive regulation of nitric-oxide synthase activity
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