Invitrogen

FABP4 Polyclonal Antibody

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FABP4 Antibody (PA5-19811) in WB

Western blot was performed using Anti-FABP4 Polyclonal Antibody (Product # PA5-19811) and a 15 kDa band corresponding to Fatty acid-binding protein, adipocyte was observed across cell lines and tissues. Whole cell extracts (40 µg lysate) of 3T3-L1 (Lane 1), 3T3-L1 differentiated to adipocytes (Lane 2), Mouse Adipose (Lane 3), Rat Adipose (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane ... View More

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FABP4 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Differentiated 3T3-L1 cells to adipocytes were transfected with Fatty acid-binding protein, adipocyte siRNA and decrease in signal intensity was observed in Western Blot application using Anti-FABP4 Polyclonal Antibody (Product # PA5-19811). {KD}

产品信息

PA5-19811

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.6 - 1 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human FABP4.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.6 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.02% sodium azide

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

AB_10983768

产品详细信息

PA5-19811 targets FABP4 in IF and WB applications and shows reactivity with Human, mouse, and Rat samples.

The PA5-19811 immunogen is synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human FABP4.

PA5-19811 detects FABP4 which has a predicted molecular weight of approximately 15 kDa.

靶标信息

FABP4 belongs to the fatty acid binding protein (FABP) family. FABPs contribute to the transport of fatty acids and other lipids in various cellular pathways. FABP4 is expressed in adipocytes, macrophages, monocyte-derived dendritic cells, and myoepithelial cells. It delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Brown Adipose Tissue Sheds Extracellular Vesicles That Carry Potential Biomarkers of Metabolic and Thermogenesis Activity Which Are Affected by High Fat Diet Intervention.

International journal of molecular sciences

Camino T,Lago-Baameiro N,Sueiro A,Bravo SB,Couto I,Santos FF,Baltar J,Casanueva FF,Pardo M

PA5-19811 was used in Western Blotting to isolate batosomes from lean and obese Sprague-Dawley rats, and to establish reference proteome maps.

Fri Sep 16 00:00:00 UTC 2022

生物信息学

蛋白别名: 3T3-L1 lipid-binding protein; A-FABP; adipocyte fatty acid binding protein; Adipocyte lipid binding protein; Adipocyte lipid-binding protein; adipocyte protein aP2; Adipocyte-type fatty acid-binding protein; AFABP; ALBP; epididymis secretory protein Li 104; Fatty acid binding protein 4 adipocyte; fatty acid binding protein 4, adipocyte; Fatty acid-binding protein 4; Fatty acid-binding protein, adipocyte; HEL S 104; Myelin P2 protein homolog; P15; P2 adipocyte protein; Protein 422

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基因别名: 422/aP2; A-FABP; AFABP; ALBP; ALBP/Ap2; Ap2; FABP4; HEL-S-104; Lbpl

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UniProt ID: (Human) P15090, (Mouse) P04117

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Entrez Gene ID: (Human) 2167, (Mouse) 11770, (Rat) 79451

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功能

transporter activity fatty acid binding lipid binding protein binding

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