Invitrogen

FAM150A Polyclonal Antibody

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2 篇参考文献

抗体检测数据 (1)
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FAM150A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM150A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FAM150A Antibody (PA5-55591) in IHC (P)

Immunohistochemical analysis of FAM150A in human prostate using FAM150A Polyclonal Antibody (Product # PA5-55591) shows moderate cytoplasmic positivity in glandular cells.

FAM150A Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FAM150A Antibody in Immunohistochemistry (IHC)

FAM150A Antibody in Western Blot (WB)

FAM150A Antibody in Western Blot (WB)

Product Image Gallery

FAM150A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of FAM150A in human prostate using FAM150A Polyclonal Antibody (Product # PA5-55591) shows moderate cytoplasmic positivity in glandular cells.

FAM150A Antibody in Immunohistochemistry (IHC) — Figure 2 Upregulation of ALKAL1 correlates with tumor malignancy and poor prognosis in colorectal cancer. (A) Representative photomicrographs of immunohistochemical analysis for ALKAL1 expression at various pathological stages, including benign, precancerous, and tumor stages I to IV. ALKAL1 was stained in brown. (B) Distribution of ALKAL1 immunostaining index across a variety of pathological stages of colorectal cancer. High expression of ALKAL1 was observed in 240/353 colorectal cancer tissue samples. (C-E) High expression of ALKAL1 was strong positive correlated with tumor classification, node classification and pathological stages. (F) Kaplan-Meier survival analysis revealed that patients with high ALKAL1 expression had poor overall survival. (G) Kaplan-Meier analysis of overall survival curves of colorectal cancer patients datasets from TCGA.

FAM150A Antibody in Western Blot (WB) — Figure 3 ALKAL1 silencing is not associated with colorectal cancer cell proliferation. (A) Real-time PCR analysis of ALKAL1 expression in ALKAL1 silencing RKO and SW480 cell lines by endogenously knocking down ALKAL1 with retrovirus (sh.#1 and sh.#2) infection. (B) Western blot analysis of ALKAL1 expression in ALKAL1 silencing RKO and SW480 cell lines by endogenously knocking down ALKAL1 with retrovirus (sh.#1 and sh.#2) infection. (C-D) CCK-8 analysis of RKO and SW480 cells proliferation. (E) Plate colony formation analysis of RKO and SW480 cell by endogenously knocking down ALKAL1 with retrovirus (sh.#1 and sh.#2) infection. (F) Cell cycle analysis with PI staining analysis of RKO and SW480 cells by endogenously knocking down ALKAL1 with retrovirus (sh.#1 and sh.#2) infection. Error bars represent the mean +- S.D. of three independent experiments. * P < 0.05.

FAM150A Antibody in Western Blot (WB) — Figure 1 ALKAL1 is upregulated in colorectal cancer tissues and cell lines. A-D) ALKAL1 expression levels was markedly upregulated in colorectal cancer tissues as assessed by analyzing TCGA colorectal cancer miRNA sequencing datasets. (E) Real-time PCR analysis of ALKAL1 in 10 primary colorectal cancer tissues compared with the matched adjacent normal tissues (ANT). (F) ALKAL1 protein expression in 10 paired CRC and adjacent normal tissues (ANT) from the same patient were detected by Western blot. alpha-tubulin was used as the loading control. (G) ALKAL1 mRNA expression was determined by RT-PCR in selected 8 colorectal cancer cell lines (Caco-2, DLD-1, HCT-8, LS 174T, RKO, SW480, SW620 and T84). (H) ALKAL1 protein expression was determined by Western blot in selected 8 colorectal cancer cell lines (Caco-2, DLD-1, HCT-8, LS 174T, RKO, SW480, SW620 and T84).

产品信息

PA5-55591

应用

建议稀释比

已发表文章

免疫印迹 (WB)

-

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免疫组化 (IHC)

-

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免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human FAM150A. Recombinant protein control fragment (Product #RP-93949).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2641273

产品详细信息

Immunogen sequence: ARVTDKEPKP LLFLPAAGAG RTPSGSRSAE IFPRDSNLKD KFIKHFTGPV TFSPECSKHF

Highest antigen sequence identity to the following orthologs: Mouse - 65%, Rat - 70%.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ALKAL1 gene silencing prevents colorectal cancer progression via suppressing Sonic Hedgehog (SHH) signaling pathway.

Journal of Cancer

Chen S,Wang B,Fu X,Liang Y,Chai X,Ye Z,Li R,He Y,Kong G,Lian J,Li X,Chen T,Zhang X,Qiu X,Tang X,Zhou K,Lin B,Zeng J

Published figure using FAM150A polyclonal antibody (Product # PA5-55591) in Western Blot

Tue Jan 05 00:00:00 UTC 2021

ALKAL1 gene silencing prevents colorectal cancer progression via suppressing Sonic Hedgehog (SHH) signaling pathway.

Journal of Cancer

Chen S,Wang B,Fu X,Liang Y,Chai X,Ye Z,Li R,He Y,Kong G,Lian J,Li X,Chen T,Zhang X,Qiu X,Tang X,Zhou K,Lin B,Zeng J

Published figure using FAM150A polyclonal antibody (Product # PA5-55591) in Western Blot

Tue Jan 05 00:00:00 UTC 2021

生物信息学

蛋白别名: ALK and LTK ligand 1; AUG-beta; Augmentor beta; family with sequence similarity 150, member A; RPLK9433

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基因别名: ALKAL1; FAM150A; UNQ9433; UNQ9433/PRO34745

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UniProt ID: (Human) Q6UXT8

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Entrez Gene ID: (Human) 389658

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protein binding receptor signaling protein tyrosine kinase activator activity receptor tyrosine kinase binding

positive regulation of neuron projection development positive regulation of protein tyrosine kinase activity positive regulation of ERK1 and ERK2 cascade positive regulation of ERK5 cascade