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FARS2 Antibody (PA5-90317) in WB
Western blot analysis of FARS2 in extracts of various cell lines. Samples were incubated with FARS2 Polyclonal antibody (Product # PA5-90317) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
产品信息
PA5-90317
产品规格
种属反应
Human,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human FARS2 (NP_0065581)
偶联物
形式
Liquid
浓度
1.33 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.01% thimerosal
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2806023
产品详细信息
Immunogen sequence: MVGSALRRGA HAYVYLVSKA SHISRGHQHQ AWGSRPPAAE CATQRAPGSV VELLGKSYPQ DDHSNLTRKV LTRVGRNLHN QQHHPLWLIK ERVKEHFYKQ YVGRFGTPLF SVYDNLSPVV TTWQNFDSLL IPADHPSRKK GDNYYLNRTH MLRAHTSAHQ WDLLHAGLDA FLVVGDVYRR DQIDSQHYPI FHQLEAVRLF SKHELFAGIK DGESLQLFEQ SSRSAHKQET HTMEAVKLVE FDLKQTLTRL; Positive Samples: A549, rat kidney; Cellular Location: Mitochondrion matrix
靶标信息
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS2 is a phenylalanine-tRNA synthetase (PheRS) localized to the mitochondrion which consists of a single polypeptide chain, unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and in the eukaryotic cytoplasm. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. FARS1 encodes a mitochondrially-located phenylalanine-tRNA synthetase (PheRS) which consists of a single polypeptide chain unlike the (alpha-beta)2 structure of the prokaryotic and eukaryotic cytoplasmic forms of PheRS. Structure analysis and catalytic properties indicate mitochondrial PheRSs may constitute a class of PheRS distinct from the enzymes found in prokaryotes and the eukaryotic cytoplasm.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: dJ236A3.1 (phenylalanine-tRNA synthetase); dJ520B18.2; dJ520B18.2 (FARS1 (phenylalanine-tRNA synthetase)); mitochondrial PHERS; pheny; phenylalanine translase; phenylalanine tRNA ligase 2, mitochondrial; Phenylalanine--tRNA ligase, mitochondrial; phenylalanine-tRNA synthetase 1 (mitochondrial); phenylalanine-tRNA synthetase 2 (mitochondrial); Phenylalanyl-tRNA synthetase; phenylalanyl-tRNA synthetase, mitochondrial; PheRS
基因别名: COXPD14; FARS1; FARS2; HSPC320; PheRS
UniProt ID: (Human) O95363, (Rat) Q6AYQ3
Entrez Gene ID: (Human) 10667, (Rat) 306879
