Invitrogen

FASN Polyclonal Antibody

FASN Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FASN Antibody (PA5-87440) in IHC (P)

Immunohistochemistry analysis of FASN in paraffin-embedded human gastric cancer. Samples were incubated with FASN Polyclonal antibody (Product # PA5-87440) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

FASN Antibody in Immunoprecipitation (IP)

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FASN Antibody — Antibody specificity was demonstrated by knockout of the target protein. Loss of signal was observed for target protein in FASN knockout (KO) 293T cell line using Anti-FASN Polyclonal Antibody (Product # PA5-87440). {KO}

产品信息

PA5-87440

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:100

酶联免疫吸附实验 (ELISA)

1 µg/mL

免疫沉淀 (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) (NP_0040954)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.9 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

AB_2804149

产品详细信息

Immunogen sequence: QQQTQLNLRS LLVNPEGPTL MRLNSVQSSE RPLFLVHPIE GSTTVFHSLA SRLSIPTYGL QCTRAAPLDS IHSLAAYYID CIRQVQPEGP YRVAGYSYGA CVAFEMCSQL QAQQSPAPTH NSLFLFDGSP TYVLAYTQSY RAKLTPGCEA EAETEAICFF VQQFTDMEHN RVLEALLPLK GLEERVAAAV DLIIKSHQGL DRQELSFAAR SFYYKLRAAE QYTPKAKYHG NVMLLRAKTG GAYGEDLGAD YNLSQVCDGK VSVHVIEGDH RTLLEGSGLE SIISIIHSSL AEPRVSVREG; Positive Samples: H460; Cellular Location: Cytoplasm, Melanosome

靶标信息

Fatty acid synthase is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.

仅用于科研。不用于诊断过程。未经明确授权不得转售。