Invitrogen

FBXW5 Polyclonal Antibody

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FBXW5 Antibody (PA5-97208) in WB

Western blot analysis of FBXW5 in extracts of A-549 cells. Samples were incubated with FBXW5 Polyclonal antibody (Product # PA5-97208) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

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产品信息

PA5-97208

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:200-1:1,000

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 184-566 of human FBXW5 (NP_0618711)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2.33 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2809010

产品详细信息

Immunogen sequence: VRNKPYDVFG CWLTETSLIS GNLHRIGDIT SCSVLWLNNA FQDVESENVN VVKRLFKIQN LNASTVRTVM VADCSRFDSP DLLLEAGDPA TSPCRIFDLG SDNEEVVAGP APAHAKEGLR HFLDRVLEGR AQPQLSERML ETKVAELLAQ GHTKPPERSA TGAKSKYLIF TTGCLTYSPH QIGIKQILPH QMTTAGPVLG EGRGSDAFFD ALDHVIDIHG HIIGMGLSPD NRYLYVNSRA WPNGAVVADP MQPPPIAEEI DLLVFDLKTM REVRRALRAH RAYTPNDECF FIFLDVSRDF VASGAEDRHG YIWDRHYNIC LARLRHEDVV NSVVFSPQEQ ELLLTASDDA TIKAWRSPRT MRVLQAPRPR PRTFFSWLAS QRR

Positive Samples: A-549; Cellular Location: Cytoplasm

靶标信息

This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains WD-40 domains, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene, however, they were found to be nonsense-mediated mRNA decay (NMD) candidates, hence not represented.

仅用于科研。不用于诊断过程。未经明确授权不得转售。