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FDX1 Antibody (MA5-50817) in ICC/IF
Immunocytochemistry analysis of FDX1 in PC-12 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with FDX1 recombinant monoclonal antibody (Product # MA5-50817) with a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the prim... View More
Product Details
MA5-50817
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
JE63-56
Immunogen
Synthetic peptide within Human ADX aa 51-100/184.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3094159
Product Specific Information
Positive control: Human placenta tissue, rat adrenal gland tissue, PC-12, SK-Br-3 cell lysate, K-562 cell lysate, HepG2 cell lysate, 293T cell lysate.
Predicted band size: 19 kDa
Subcellular Location: Mitochondrion matrix.
Target Information
The product of this gene is a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to a terminal cytochrome P450. This particular oxidation/reduction system is found in steroidogenic tissues, and is involved with the synthesis of bile acid and vitamin D. In addition to the expressed gene at this chromosomal locus (11q22), there are pseudogenes located on chromosomes 20 and 21. This gene product has been identified in a number of different tissues but all forms have been shown to be identical and are not tissue specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adrenal ferredoxin; Adrenodoxin, mitochondrial; Ferredoxin-1; Hepatoredoxin; mitochondrial adrenodoxin
Gene Aliases: ADX; FDX; FDX1; LOH11CR1D
UniProt ID: (Human) P10109, (Rat) P24483, (Mouse) P46656
Entrez Gene ID: (Human) 2230, (Rat) 29189, (Mouse) 14148
C21-steroid hormone biosynthetic process
cholesterol metabolic process
sterol metabolic process
hormone biosynthetic process
small molecule metabolic process
oxidation-reduction process
steroid biosynthetic process
adrenal gland development
response to gonadotropin
cellular response to leptin stimulus
response to leptin
regulation of catalytic activity
positive regulation of oxidoreductase activity
NADPH oxidation
cellular response to organic cyclic compound
cellular response to epinephrine stimulus
lipid metabolic process
transport
steroid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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