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FER Antibody (MA5-15357) in ICC/IF
Immunofluorescence analysis of FER was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FER Mouse Monoclonal Antibody (5D2C4) (Product # MA5-15357) at 1: 100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28... View More
产品信息
MA5-15357
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
5D2C4
抗原
Purified recombinant fragment of human FER expressed in E. Coli.
偶联物
形式
Liquid
浓度
Conc. Not Determined
保存液
ascites
内含物
0.03% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_10981288
产品详细信息
MA5-15357 targets FER in indirect ELISA, IF, IHC, and WB applications and shows reactivity with Human and mouse samples.
The MA5-15357 immunogen is purified recombinant fragment of human FER expressed in E. Coli.
MA5-15357 detects FER which has a predicted molecular weight of approximately 95kDa.
靶标信息
FER is a member of the FPS/FES family of non-receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. FER is found in the cytoplasm and in the nucleus, where it was associated with the chromatin fraction. Its ubiquitous expression and its subcellular location indicate that it may be involved in key regulatory processes. The group IV cytoplasmic protein-tyrosine kinase Fer has been linked to cellular signaling responses to many different stimuli, including growth factors and cytokines. Data suggest a biological role for Fer in regulation of leukocyte recruitment during the innate immune response.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: c-FER; EC 2.7.10.2; Feline encephalitis virus-related kinase FER; fer (fms/fps related) protein kinase, testis specific 2; fer (fps/fes related) tyrosine kinase; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; p47; p94-FER; phosphoprotein NCP94; protein phosphatase 1, regulatory subunit 74; Proto-oncogene c-Fer; proto-oncogene tyrosine-protein kinase FER; Tyrosine kinase 3; tyrosine protein kinase FLK; Tyrosine-protein kinase Fer; Tyrosine-protein kinase FLK
基因别名: AV082135; C330004K01Rik; FER; Fert; Fert2; Flk; Flk_retired; p94-Fer; PPP1R74; TYK3
UniProt ID: (Human) P16591, (Mouse) P70451, (Rat) P09760
Entrez Gene ID: (Human) 2241, (Mouse) 14158, (Rat) 301737
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
epidermal growth factor receptor binding
protein binding
ATP binding
protein phosphatase 1 binding
lipid binding
nucleotide binding
actin binding
protein kinase activity
receptor binding
cytoskeletal protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rab GTPase binding
protein kinase binding
gamma-catenin binding
cadherin binding
cell adhesion molecule binding
microtubule cytoskeleton organization
mitotic cell cycle
regulation of protein phosphorylation
protein phosphorylation
chemotaxis
cell adhesion
signal transduction
cell proliferation
regulation of lamellipodium assembly
regulation of fibroblast migration
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
cell differentiation
positive regulation of cell migration
positive regulation of actin filament polymerization
actin cytoskeleton reorganization
response to lipopolysaccharide
cellular response to insulin stimulus
negative regulation of mast cell activation involved in immune response
substrate adhesion-dependent cell spreading
cellular response to reactive oxygen species
extracellular matrix-cell signaling
cellular response to macrophage colony-stimulating factor stimulus
response to platelet-derived growth factor
insulin receptor signaling pathway via phosphatidylinositol 3-kinase
peptidyl-tyrosine autophosphorylation
Fc-epsilon receptor signaling pathway
Kit signaling pathway
regulation of epidermal growth factor receptor signaling pathway
regulation of cell proliferation
tyrosine phosphorylation of Stat3 protein
regulation of mast cell degranulation
cell-cell adhesion mediated by cadherin
innate immune response
protein autophosphorylation
platelet-derived growth factor receptor signaling pathway
diapedesis
positive regulation of NF-kappaB transcription factor activity
interleukin-6-mediated signaling pathway
epidermal growth factor receptor signaling pathway
cell migration
positive regulation of cell proliferation
intracellular signal transduction
