Invitrogen

FGF4 Polyclonal Antibody, Biotin, PeproTech®

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FGF4 Antibody (500-P158BT-25UG) in ELISA

Sandwich ELISA: To detect hFGF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of FGF4 Polyclonal Antibody, Biotin (Product # 500-P158BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech FGF4 Polyclonal Antibody (Product # 500-P158-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hFGF-4.

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产品信息

500-P158BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human FGF-4

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偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929544

产品详细信息

AA Sequence of recombinant protein: GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-4. Anti-Human FGF-4-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hFGF-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FGF-4 (500-P158) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hFGF-4.

Western Blot: To detect hFGF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hFGF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P158BT-1MG will be provided as 2 x 500 µg

靶标信息

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FGF; FGF-4; Fibroblast growth factor; Fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; oncogene HST; Transforming protein KS3

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基因别名: FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF; KS3

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UniProt ID: (Human) P08620

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Entrez Gene ID: (Human) 2249

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功能

protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding growth factor activity heparin binding 1-phosphatidylinositol-3-kinase activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity growth factor intercellular signal molecule

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