Invitrogen

FGF4 Recombinant Rabbit Monoclonal Antibody (2H7L18)

FGF4 Antibody in Immunocytochemistry (ICC/IF)

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FGF4 Antibody (703064) in ICC/IF

For immunofluorescence analysis, induced pluripotent stem (iPS) cells were fixed and permeabilized for detection of endogenous FGF4 using Anti-FGF4 Recombinant Rabbit Monoclonal Antibody (Product # 703064, 1:100) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). Panel a-c) clearly demonstrates cytoplasmic localization of FGF4 in iPS cells. Panel (d-f) sho... View More

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FGF4 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Expression of FGF4 was observed specifically in iPSCs in comparison to Embryoid bodies using Anti-FGF4 Recombinant Rabbit Monoclonal Antibody (Product # 703064) in Immunofluorescence. {RE}

产品信息

703064

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

2H7L18

抗原

Protein corresponding to human FGF4 [aa79-aa206]

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2784557

产品详细信息

This antibody is predicted to react with Monkey, Dog, Pig.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FGF; FGF-4; Fibroblast growth factor; Fibroblast growth factor 4; fibroblast growth factor 4 splice isoform; HBGF-4; heparin secretory transforming protein 1; Heparin secretory-transforming protein 1; Heparin-binding growth factor 4; HST; HSTF-1; human stomach cancer, transforming factor from FGF-related oncogene; kaposi sarcoma oncogene; oncogene HST; Transforming protein KS3

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基因别名: FGF4; HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF; KS3

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UniProt ID: (Human) P08620

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Entrez Gene ID: (Human) 2249

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功能

protein tyrosine kinase activity Ras guanyl-nucleotide exchange factor activity fibroblast growth factor receptor binding growth factor activity heparin binding 1-phosphatidylinositol-3-kinase activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity growth factor intercellular signal molecule

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