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FGF9 Antibody (500-P66-1MG) in WB
Western Blot: To detect mFGF-9 by Western Blot analysis FGF9 Polyclonal Antibody (Product # 500-P66-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mFGF-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
产品信息
500-P66-1MG
产品规格
种属反应
Mouse
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived Recombinant Murine FGF-9
偶联物
形式
Lyophilized
浓度
0.1-1.0 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS
内含物
no preservative
保存条件
-20°C
运输条件
Ambient
RRID
AB_2930690
产品详细信息
AA Sequence of recombinant protein: PLGEVGSYFG VQDAVPFGNV PVLPVDSPVL LNDHLGQSEA GGLPRGPAVT DLDHLKGILR RRQLYCRTGF HLEIFPNGTI QGTRKDHSRF GILEFISIAV GLVSIRGVDS GLYLGMNEKG ELYGSEKLTQ ECVFREQFEE NWYNTYSSNL YKHVDTGRRY YVALNKDGTP REGTRTKRHQ KFTHFLPRPV DPDKVPELYK DILSQS.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine FGF-9. Anti-Murine FGF-9-specific antibody was purified by affinity chromatography employing an immobilized Murine FGF-9 matrix.
Sandwich ELISA: To detect Murine FGF-9 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine FGF-9 (500-P66Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine FGF-9.
Western Blot: To detect mFGF-9 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant mFGF-9 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
靶标信息
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. Expression of the mouse homolog of this gene was found to be dependent on Sonic hedgehog (Shh) signaling. Mice lacking the homolog gene displayed a male-to-female sex reversal phenotype, which suggested a role in testicular embryogenesis.
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生物信息学
蛋白别名: elbow knee synostosis; FGF; FGF-9; Fibroblast growth factor; Fibroblast growth factor 9; GAF; glia activating factor; Glia-activating factor; HBGF-9; M-FGF-9
基因别名: Eks; Fgf-9; Fgf9
UniProt ID: (Mouse) P54130
Entrez Gene ID: (Mouse) 14180
negative regulation of transcription from RNA polymerase II promoter
angiogenesis
osteoblast differentiation
positive regulation of mesenchymal cell proliferation
chondrocyte differentiation
cardiac left ventricle morphogenesis
cardiac ventricle development
protein import into nucleus
cell-cell signaling
multicellular organism development
positive regulation of cell proliferation
fibroblast growth factor receptor signaling pathway
male gonad development
positive regulation of gene expression
cell differentiation
negative regulation of Wnt signaling pathway
male sex determination
lung development
embryonic limb morphogenesis
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of activin receptor signaling pathway
inner ear morphogenesis
positive regulation of MAPK cascade
positive regulation of smoothened signaling pathway
positive regulation of transcription, DNA-templated
regulation of timing of cell differentiation
embryonic digestive tract development
embryonic skeletal system development
positive regulation of epithelial cell proliferation
positive regulation of cell division
positive regulation of cardiac muscle cell proliferation
lung-associated mesenchyme development
vasculogenesis involved in coronary vascular morphogenesis
positive regulation of canonical Wnt signaling pathway
