Invitrogen

FHIT Polyclonal Antibody

FHIT Antibody in Immunocytochemistry (ICC/IF)

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FHIT Antibody (71-9000) in ICC/IF

Immunofluorescence analysis of FHIT Polyclonal Antibody was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FHIT Rabbit Polyclonal Antibody at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More

FHIT Antibody in Immunohistochemistry (IHC)

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产品信息

71-9000

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-3 µg/mL

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免疫组化 (IHC)

Assay-dependent

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免疫细胞化学 (ICC/IF)

2 µg/mL

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酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Rat

已发表种属

Human, Mouse, Virus

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Full length human FHIT fusion protein with GST (glutathione amino transferase)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2534004

靶标信息

Alterations and deletions of the FHIT gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT may act as a tumor suppressor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Fhit induces the reciprocal suppressions between Lin28/Let-7 and miR-17/92miR.

International journal of medical sciences

Chae HJ,Seo JB,Kim SH,Jeon YJ,Suh SS

Published figure using FHIT polyclonal antibody (Product # 71-9000) in Western Blot

Fri Sep 17 00:00:00 UTC 2021

Restoration of MHC-I on Tumor Cells by Fhit Transfection Promotes Immune Rejection and Acts as an Individualized Immunotherapeutic Vaccine.

Cancers

Pulido M,Chamorro V,Romero I,Algarra I,S-Montalvo A,Collado A,Garrido F,Garcia-Lora AM

71-9000 was used in Immunohistochemistry to determine the effect of transfecting Fhit in another MHC-I-negative tumor clone of the GR9 mouse tumor model, and to examine the effect of Fhit transfection and MHC-I expression restoration on the biological, oncogenic, and immunogenic characteristics of MHC-I negative tumor cells.

Fri Jun 12 00:00:00 UTC 2020

Restoration of MHC-I on Tumor Cells by Fhit Transfection Promotes Immune Rejection and Acts as an Individualized Immunotherapeutic Vaccine.

Cancers

Pulido M,Chamorro V,Romero I,Algarra I,S-Montalvo A,Collado A,Garrido F,Garcia-Lora AM

Fri Jun 12 00:00:00 UTC 2020

Upregulation of miR-130b Contributes to Risk of Poor Prognosis and Racial Disparity in African-American Prostate Cancer.

Cancer prevention research (Philadelphia, Pa.)

Hashimoto Y,Shiina M,Dasgupta P,Kulkarni P,Kato T,Wong RK,Tanaka Y,Shahryari V,Maekawa S,Yamamura S,Saini S,Deng G,Tabatabai ZL,Majid S,Dahiya R

71-9000 was used in Immunohistochemistry-immunofluorescence to elucidate the role of miR-130b as a contributor to prostate cancer health disparity in African-American patients.

Sun Sep 01 00:00:00 UTC 2019

Upregulation of miR-130b Contributes to Risk of Poor Prognosis and Racial Disparity in African-American Prostate Cancer.

Cancer prevention research (Philadelphia, Pa.)

Hashimoto Y,Shiina M,Dasgupta P,Kulkarni P,Kato T,Wong RK,Tanaka Y,Shahryari V,Maekawa S,Yamamura S,Saini S,Deng G,Tabatabai ZL,Majid S,Dahiya R

71-9000 was used in Immunohistochemistry-immunofluorescence to elucidate the role of miR-130b as a contributor to prostate cancer health disparity in African-American patients.

Sun Sep 01 00:00:00 UTC 2019

Fhit regulates EMT targets through an EGFR/Src/ERK/Slug signaling axis in human bronchial cells.

Molecular cancer research : MCR

Joannes A,Grelet S,Duca L,Gilles C,Kileztky C,Dalstein V,Birembaut P,Polette M,Nawrocki-Raby B

71-9000 was used in western blot to investigate the mechanism of fragile histidine triad-mediated regulation of epithelial-mesenchymal transition.

Thu May 01 00:00:00 UTC 2014

Reduced FHIT expression is associated with mismatch repair deficient and high CpG island methylator phenotype colorectal cancer.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Al-Temaimi RA,Jacob S,Al-Ali W,Thomas DA,Al-Mulla F

71-9000 was used in immunohistochemistry to study the fragile histidine triad gene in microsatellite instable, microsatellite stable, and CpG island methylator phenotypes of colorectal cancer subtypes.

Sun Sep 01 00:00:00 UTC 2013

Differential protein immunoexpression profiles in appendiceal mucinous neoplasms: a special reference to classification and predictive factors.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

Yoon SO,Kim BH,Lee HS,Kang GH,Kim WH,Kim YA,Kim JE,Chang MS

Sat Aug 01 00:00:00 UTC 2009

Increased sensitivity to cisplatin in non-small cell lung cancer cell lines after FHIT gene transfer.

Neoplasia (New York, N.Y.)

Andriani F,Perego P,Carenini N,Sozzi G,Roz L

Published figure using FHIT polyclonal antibody (Product # 71-9000) in Western Blot

Sun Jan 01 00:00:00 UTC 2006

Protein expression profiling identifies subclasses of breast cancer and predicts prognosis.

Cancer research

Jacquemier J,Ginestier C,Rougemont J,Bardou VJ,Charafe-Jauffret E,Geneix J,Adélaïde J,Koki A,Houvenaeghel G,Hassoun J,Maraninchi D,Viens P,Birnbaum D,Bertucci F

Tue Feb 01 00:00:00 UTC 2005

Synergistic tumor suppression by coexpression of FHIT and p53 coincides with FHIT-mediated MDM2 inactivation and p53 stabilization in human non-small cell lung cancer cells.

Cancer research

Nishizaki M,Sasaki J,Fang B,Atkinson EN,Minna JD,Roth JA,Ji L

71-9000 was used in Immunocytochemistry-immunoflourescence to study the combined effects of FHIT and p53 proteins on tumour cell proliferation and apoptosis in non-small cell lung carcinoma cells, and on tumour growth.

Sun Aug 15 00:00:00 UTC 2004

Synergistic tumor suppression by coexpression of FHIT and p53 coincides with FHIT-mediated MDM2 inactivation and p53 stabilization in human non-small cell lung cancer cells.

Cancer research

Nishizaki M,Sasaki J,Fang B,Atkinson EN,Minna JD,Roth JA,Ji L

Sun Aug 15 00:00:00 UTC 2004

The apoptotic pathway triggered by the Fhit protein in lung cancer cell lines is not affected by Bcl-2 or Bcl-x(L) overexpression.

Oncogene

Roz L,Andriani F,Ferreira CG,Giaccone G,Sozzi G

71-9000 was used in western blot to elucidate how fragile histidine triad proteins suppress the development of cancer

Thu Dec 02 00:00:00 UTC 2004

Loss of fragile histidine triad protein in human hepatocellular carcinoma.

World journal of gastroenterology

Zhao P,Song X,Nin YY,Lu YL,Li XH

Sun Jun 01 00:00:00 UTC 2003

Alterations of the fragile histidine triad gene, FHIT, and its encoded products contribute to testicular germ cell tumorigenesis.

Cancer research

Kraggerud SM,Aman P,Holm R,Stenwig AE,Fosså SD,Nesland JM,Lothe RA

Tue Jan 15 00:00:00 UTC 2002

Restoration of fragile histidine triad (FHIT) expression induces apoptosis and suppresses tumorigenicity in lung and cervical cancer cell lines.

Proceedings of the National Academy of Sciences of the United States of America

Roz L,Gramegna M,Ishii H,Croce CM,Sozzi G

Published figure using FHIT polyclonal antibody (Product # 71-9000) in Western Blot

Tue Mar 19 00:00:00 UTC 2002

Restoration of fragile histidine triad (FHIT) expression induces apoptosis and suppresses tumorigenicity in lung and cervical cancer cell lines.

Proceedings of the National Academy of Sciences of the United States of America

Roz L,Gramegna M,Ishii H,Croce CM,Sozzi G

Published figure using FHIT polyclonal antibody (Product # 71-9000) in Western Blot

Tue Mar 19 00:00:00 UTC 2002

生物信息学

蛋白别名: Adenosine 5'-monophosphoramidase FHIT; Adenylylsulfatase; Adenylylsulfate-ammonia adenylyltransferase; AP3A hydrolase; AP3Aase; Bis(5'-adenosyl)-triphosphatase; bis5'-adenosyl-triphosphatase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein

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基因别名: AP3Aase; FHIT; FRA3B

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UniProt ID: (Human) P49789, (Rat) Q9JIX3

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Entrez Gene ID: (Human) 2272, (Rat) 60398

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功能

nucleotide binding catalytic activity protein binding hydrolase activity ubiquitin protein ligase binding identical protein binding bis(5'-adenosyl)-triphosphatase activity nickel cation binding phosphatase

参与通路

purine nucleotide metabolic process transcription, DNA-templated regulation of transcription, DNA-templated nucleotide metabolic process negative regulation of proteasomal ubiquitin-dependent protein catabolic process intrinsic apoptotic signaling pathway by p53 class mediator DNA replication diadenosine triphosphate catabolic process