Invitrogen

FMN1 Polyclonal Antibody

抗体检测数据 (3)

FMN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FMN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FMN1 Antibody (PA5-79275) in IHC (P)

Immunohistochemistry analysis of FMN1 on paraffin-embedded mouse testis tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with FMN1 polyclonal antibody (Product# PA5-79275) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Developme... View More

FMN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FMN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FMN1 Antibody in Western Blot (WB)

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FMN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FMN1 on paraffin-embedded mouse testis tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with FMN1 polyclonal antibody (Product# PA5-79275) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

FMN1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of FMN1 on paraffin-embedded rat testis tissue. Antigen retrieval was performed using citrate buffer (pH6, epitope retrieval solution) for 20 mins. Sample was blocked using 10% goat serum, incubated with FMN1 polyclonal antibody (Product# PA5-79275) with a dilution of 1 µg/mL (overnight at 4°C), and followed by biotinylated goat anti-rabbit IgG (30 minutes at 37°C). Development was performed using Streptavidin-Biotin-Complex (SABC) with DAB chromogen method.

FMN1 Antibody in Western Blot (WB) — Western blot analysis of FMN1 in Lane 1: rat brain tissue lysate, Lane 2: mouse brain tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with FMN1 polyclonal antibody (Product # PA5-79275) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10,000. Signal development was performed using a chemiluminescence (ECL) kit.

产品信息

PA5-79275

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E. coli-derived human FMN1 recombinant protein (Position: N1195-N1419).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2746391

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Plays a role in the formation of adherens junction and the polymerization of linear actin cables.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: formin (limb deformity); formin 1; Formin-1; Limb deformity protein; Limb deformity protein homolog

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基因别名: FMN; FMN1; LD; RGD1306349; RGD1310100

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UniProt ID: (Human) Q68DA7, (Mouse) Q05860

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Entrez Gene ID: (Human) 342184, (Rat) 296512, (Mouse) 14260

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actin binding microtubule binding SH3 domain binding protein binding

参与通路

gene expression forelimb morphogenesis hindlimb morphogenesis actin nucleation skeletal system morphogenesis dendrite morphogenesis positive regulation of actin nucleation positive regulation of focal adhesion assembly ureteric bud invasion kidney development positive regulation of actin filament polymerization limb development