Invitrogen

FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02)

FOXA1 Antibody in Immunocytochemistry (ICC/IF)

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FOXA1 Antibody (MA5-32556) in ICC/IF

Immunofluorescence analysis of FOXA1 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02) (Product # MA5-32556) at 1:50-1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Flu... View More

FOXA1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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FOXA1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across MCF7, T-47D, LNCaP, SW480, HCT-116 and SHSY-5Y owing to their inherent genetic constitution. Relative expression of Ci-fkh; fkd7; fkh; fork head domain; forkhead box A1; forkhead box protein A1; forkhead-7; Foxa1; FoxA4a; hepatocyte nuclear factor 3 alpha (winged helix transcription factor); hepatocyte nuclear factor 3, alpha; hepatocyte nuclear factor 3-alpha; HNF3A; HNF-3A; HNF3alpha; HNF-3-alpha; HNF3-alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF3A; Tcf-3a; tFoxA1; Transcription factor 3A; xfkh1; xfkh2 was observed in MCF7, T-47D, LNCaP and SW480 using Anti-FOXA1 Recombinant Rabbit Monoclonal Antibody (JF10-02) (Product # MA5-32556) in Western Blot. {RE}

产品信息

MA5-32556

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JF10-02

抗原

Recombinant protein within Human FOXA1 aa 320-472

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2809833

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

This gene encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Ci-fkh; fkd7; fkh; fork head domain; Forkhead box protein A1; forkhead-7; FoxA4a; hepatocyte nuclear factor 3 alpha (winged helix transcription factor); hepatocyte nuclear factor 3, alpha; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; HNF3-alpha; HNF3alpha; MGC33105; MGC52590; MGC75967; pintallavis; TCF-3A; tFoxA1; Transcription factor 3A; xfkh1; xfkh2

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基因别名: FOXA1; Hnf-3a; HNF3A; Tcf-3a; TCF3A

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UniProt ID: (Human) P55317, (Mouse) Q4VA63, (Rat) P23512

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Entrez Gene ID: (Human) 3169, (Mouse) 15375, (Rat) 25098

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功能