Invitrogen

FOXA3 Polyclonal Antibody

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FOXA3 Antibody (PA1-813) in WB

Western blot analysis of FOXA3 was performed by loading 25 µg of HepG2 (lane 1) mouse liver (lane 2) and rat liver (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a FOXA3 polyclonal antibody (Product # PA1-813) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotti... View More

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产品信息

PA1-813

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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染色质免疫沉淀实验 (ChIP)

1-3 µL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues S(4) V K M E A H D L A E W S Y Y P E A G E(23) of mouse FOXA3.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2105002

产品详细信息

PA1-813 detects FOXA3 from human samples.

PA1-813 has been successfully used in Western blot and ChIP applications. By Western blot, PA1-813 detects a ~37 kDa band representing FOXA3 from mouse liver.

The PA1-813 immunogen is a synthetic peptide corresponding to residues S(4) V K M E A H D L A E W S Y Y P E A G E(23) of mouse FOXA3. This immunizing peptide (PEP-299) is available for use in neutralization and control experiments.

靶标信息

FOXA3 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. The crystal structure of a similar protein in rat has been resolved.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Impact of Fetal Exposure to Endocrine Disrupting Chemical Mixtures on FOXA3 Gene and Protein Expression in Adult Rat Testes.

International journal of molecular sciences

Walker C,Boisvert A,Malusare P,Culty M

PA1-813 was used in Chromatin immunoprecipitation to show that FOXA3 downregulation in adult testes following fetal exposure to Gen + DEHP may contribute to adverse male reproductive outcomes.

Sat Jan 07 00:00:00 UTC 2023

Hepatocyte-like cells generated by direct reprogramming from murine somatic cells can repopulate decellularized livers.

Biotechnology and bioengineering

Chen C,Pla-Palacín I,Baptista PM,Shang P,Oosterhoff LA,van Wolferen ME,Penning LC,Geijsen N,Spee B

PA1-813 was used in Western Blotting to develop a polycistronic expression system to induce the direct reprogramming of mouse embryonic fibroblasts towards hepatocytes.

Thu Nov 01 00:00:00 UTC 2018

生物信息学

蛋白别名: Fork head-related protein FKH H3; Forkhead box protein A3; hepatocyte nuclear factor 3 gamma (winged helix transcription factor); hepatocyte nuclear factor 3, gamma; Hepatocyte nuclear factor 3-gamma; HNF-3-gamma; HNF-3G; TCF-3G; Transcription factor 3G

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基因别名: FKHH3; FOXA3; Hnf-3g; HNF3G; Tcf-3g; TCF3G

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UniProt ID: (Human) P55318, (Mouse) P35584, (Rat) P32183

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Entrez Gene ID: (Human) 3171, (Mouse) 15377, (Rat) 25100

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功能

RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein domain specific binding sequence-specific DNA binding transcription regulatory region DNA binding DNA binding

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