Invitrogen

FOXA3 Polyclonal Antibody

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4 篇参考文献

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抗体检测数据 (3)
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FOXA3 Antibody in Immunohistochemistry (IHC)

FOXA3 Antibody in Immunohistochemistry (IHC)

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FOXA3 Antibody (PA5-106980) in IHC

Immunohistochemistry of FOXA3 in human lung using a FOXA3 Polyclonal Antibody (Product # PA5-106980).

FOXA3 Antibody in Immunohistochemistry (IHC)

FOXA3 Antibody in Western Blot (WB)

FOXA3 Antibody in Western Blot (WB)

FOXA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FOXA3 Antibody in Western Blot (WB)

FOXA3 Antibody in Western Blot (WB)

Product Image Gallery

FOXA3 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry of FOXA3 in human lung using a FOXA3 Polyclonal Antibody (Product # PA5-106980).

FOXA3 Antibody in Western Blot (WB) — Western Blot analysis of FOXA3 in mouse skeletal muscle using a FOXA3 Polyclonal Antibody (Product # PA5-106980) diluted to 0.2-1 µg/mL.

FOXA3 Antibody in Western Blot (WB) — Western Blot analysis of FOXA3 in human Jurkat cells using a FOXA3 Polyclonal Antibody (Product # PA5-106980) diluted to 0.2-1 µg/mL.

FOXA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Knockdown of HOXC10 suppresses ESCC tumorigenesis in vivo . (A) The murine tumor images in the shCtrl and shHOXC10-1 groups (n = 5). (B) Mouse tumor size curves and (C) weight in the two groups (n = 5). (D) HE staining was conducted to evaluate the morphological changes in mouse tumor tissues. (E) IHC assays were used to measure the protein expression of Ki-67, HOXC10, and FOXA3 in mouse tumors. All experiments were triplicated. Scale bar = 50 mum **P < 0.01 , ***P < 0.001 . Fig. 6

FOXA3 Antibody in Western Blot (WB) — Fig. 4 HOXC10 binds with FOXA3 in ESCC cells. (A) The interaction between HOXC10 and FOXA3 proteins is predicted by the String database. (B) GEPIA website showed a correlation between HOXC10 and FOXA3 in esophageal carcinoma. (C) GEPIA database presented the expression pattern of FOXA3 in esophageal carcinoma. (D) The survival curves of esophageal carcinoma patients with different FOXA3 expression on the Kaplan-Meier Plotter database ( http://kmplot.com/analysis/ ). (E) qRT-PCR and (F) Western blot were used to measured FOXA3 expression in ESCC patient samples and NTs. (G) qRT-PCR detected FOXA3 mRNA level in ESCC cell lines. (H) Co-IP assays evaluated the biding of FOXA3 with HOXC10 in ESCC cells. (I) Western blot detected FOXA3 protein expression in ESCC cells with HOXC10-silenced. All experiments were triplicated. **P < 0.01 , ***P < 0.001 . Fig. 4

FOXA3 Antibody in Western Blot (WB) — Fig. 5 HOXC10 enhances ESCC cell malignancy by upregulating FOXA3. (A) qRT-PCR and (B) Western blot measured FOXA3 overexpression efficacy in ESCC cells. (C) ESCC cell after silencing HOXC10 and/or overexpressing FOXA3 was evaluated using CCK-8 assays. (D) Transwell and (E) wound scratch assays were used to assess invasiveness and migrating capacity of ESCC cells. (F) Flow cytometry was used toexamine apoptosis in ESCC cells after the indicated transfections. All experiments were triplicated. Scale bar = 50 mum **P < 0.01 , ***P < 0.001 ; ###P < 0.001 . Fig. 5

产品信息

PA5-106980

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.2-1 µg/mL

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免疫组化 (IHC)

0.2-1 µg/mL

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免疫组化（石蜡） (IHC (P))

-

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免疫沉淀 (IP)

-

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide directed towards the middle region of human FOXA3 (aa 201-250)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.4, with 2% sucrose

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2817696

产品详细信息

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 92%; Human: 100%; Mouse: 92%; Rabbit: 85%; Rat: 100%; Zebrafish: 79%

Immunogen sequence: FENGCYLRRQ KRFKLEEKVK KGGSGAATTT RNGTGSAAST TTPAATVTSP

靶标信息

FOXA3 encodes a member of the forkhead class of DNA-binding proteins. These hepatocyte nuclear factors are transcriptional activators for liver-specific transcripts such as albumin and transthyretin, and they also interact with chromatin. Similar family members in mice have roles in the regulation of metabolism and in the differentiation of the pancreas and liver. The crystal structure of a similar protein in rat has been resolved.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Immunohistochemistry to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Immunohistochemistry to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Immunohistochemistry to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Immunohistochemistry to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

HOXC10 promotes esophageal squamous cell carcinoma progression by targeting FOXA3 and indicates poor survival outcome.

Heliyon

He X,Wang H,Wang R,Li Y,Li S,Cao X,Xu J

PA5-106980 was used in Immunohistochemistry to support a key role for HOXC10 in the tumorigenesis of ESCC by upregulating FOXA3 via the MAPK pathway and highlight its potential as a promising diagnostic and prognostic marker for ESCC.

Sun Oct 01 00:00:00 UTC 2023

生物信息学

蛋白别名: Fork head-related protein FKH H3; Forkhead box protein A3; hepatocyte nuclear factor 3 gamma (winged helix transcription factor); hepatocyte nuclear factor 3, gamma; Hepatocyte nuclear factor 3-gamma; HNF-3-gamma; HNF-3G; TCF-3G; Transcription factor 3G

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基因别名: FKHH3; FOXA3; Hnf-3g; HNF3G; Tcf-3g; TCF3G

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UniProt ID: (Human) P55318, (Mouse) P35584

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Entrez Gene ID: (Human) 3171, (Mouse) 15377

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RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding transcription factor binding protein domain specific binding sequence-specific DNA binding transcription regulatory region DNA binding DNA binding winged helix/forkhead transcription factor

cellular glucose homeostasis transcription, DNA-templated multicellular organism development spermatogenesis cellular response to starvation covalent chromatin modification cell differentiation positive regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter negative regulation of cell proliferation anatomical structure morphogenesis chromatin modification positive regulation of hepatocyte differentiation