Invitrogen

FOXC1 Polyclonal Antibody

FOXC1 Antibody in Immunocytochemistry (ICC/IF)

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FOXC1 Antibody (PA1-807) in ICC/IF

Immunofluorescence analysis of FOXC1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FOXC1 Rabbit Polyclonal Antibody (Product # PA1-807) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a... View More

FOXC1 Antibody in Western Blot, ChIP Assay (WB, ChIP)

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FOXC1 Antibody — Antibody specificity was demonstrated through detection of enrichment of the target protein at specific gene loci. Chromatin Immunoprecipitation (ChIP) was performed using anti-FOXC1 rabbit polyclonal antibody (catalog # PA1-807), with relevant positive (FGF) and negative target genes/ binding sites(SAT2) {RE}

产品信息

PA1-807

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

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免疫细胞化学 (ICC/IF)

2 µg/mL

染色质免疫沉淀实验 (ChIP)

1-3 µg x 10^6 cells

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产品规格

种属反应

Human, Mouse

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues A(423) V D D P L P D Y S L P(434) of human FOXC1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2247078

产品详细信息

PA1-807 detects FOXC1 from human and mouse samples.

PA1-807 has been successfully used in Western blot procedures. By Western blot, PA1-807 detects a ~50 kDa band representing FOXC1 from HepG2 cells and mouse kidney cells. This antibody also detects a nonspecific band at ~45 kDa from HepG2 cells, and at ~60 kDa from mouse kidney samples.

The PA1-807 immunogen is a synthetic peptide corresponding to residues A(423) V D D P L P D Y S L P(434) of human FOXC1.

PA1-807 can be used with blocking peptide PEP-298.

靶标信息

FOXC1 is a protein belonging to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this protein is not known, however, it plays a role in the regulation of FGF19-FGFR4-MAPK pathway to promote both the development and maintenance of anterior segment structures within the eye. Mutations in this gene cause various glaucoma, iridogoniodysgenesis anomaly, Peters anomaly that includes central corneal leukoma, absence of the posterior corneal stroma and Descemetmembrane and Axenfeld-Rieger anomaly characterized by posterior corneal embryotoxon, iris adhesion to the Schwalbe line, hypertelorism, hypoplasia of the malar bones, congenital absence of some teeth and mental retardation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Role of stemness-related genes TIMP1, PGF, and SNAI1 in the prognosis of colorectal cancer through single-cell RNA-seq.

Cancer medicine

Shen Y,Ni S,Li S,Lv B

PA1-807 was used in Western Blotting to analyze stemness-related prognostic genes of CRC based on single-cell RNA-sequencing (scRNA-seq) data.

Mon May 01 00:00:00 UTC 2023

Role of stemness-related genes TIMP1, PGF, and SNAI1 in the prognosis of colorectal cancer through single-cell RNA-seq.

Cancer medicine

Shen Y,Ni S,Li S,Lv B

PA1-807 was used in Western Blotting to analyze stemness-related prognostic genes of CRC based on single-cell RNA-sequencing (scRNA-seq) data.

Mon May 01 00:00:00 UTC 2023

Single-cell sequencing reveals activation of core transcription factors in PRC2-deficient malignant peripheral nerve sheath tumor.

Cell reports

Zhang X,Lou HE,Gopalan V,Liu Z,Jafarah HM,Lei H,Jones P,Sayers CM,Yohe ME,Chittiboina P,Widemann BC,Thiele CJ,Kelly MC,Hannenhalli S,Shern JF

PA1-807 was used in ChIP assay to define the oncogenic mechanisms underlying PRC2 loss, and discover a two-pronged oncogenic process.

Tue Sep 20 00:00:00 UTC 2022

Testicular orphan receptor 4 (TR4) promotes papillary thyroid cancer invasion via activating circ-FNLA/miR-149-5p/MMP9 signaling.

Molecular therapy. Nucleic acids

Ouyang X,Feng L,Yao L,Xiao Y,Hu X,Zhang G,Liu G,Wang Z

PA1-807 was used in Western Blot to investigate the oncogenic role of testicular orphan receptor 4 in papillary thyroid cancer development.

Fri Jun 04 00:00:00 UTC 2021

System analysis of the regulation of the immune response by CD147 and FOXC1 in cancer cell lines.

Oncotarget

Shang YK,Li C,Liu ZK,Kong LM,Wei D,Xu J,Wang ZL,Bian H,Chen ZN

Published figure using FOXC1 polyclonal antibody (Product # PA1-807) in ChIP assay

Tue Feb 27 00:00:00 UTC 2018

System analysis of the regulation of the immune response by CD147 and FOXC1 in cancer cell lines.

Oncotarget

Shang YK,Li C,Liu ZK,Kong LM,Wei D,Xu J,Wang ZL,Bian H,Chen ZN

Published figure using FOXC1 polyclonal antibody (Product # PA1-807) in ChIP assay

Tue Feb 27 00:00:00 UTC 2018

生物信息学

蛋白别名: congenital hydrocephalus; forkhead box C1 protein; Forkhead box protein C1; forkhead, drosophila, homolog-like 7; forkhead-related activator 3; Forkhead-related protein FKHL7; Forkhead-related transcription factor 3; forkhead/winged helix-like transcription factor 7; FREAC-3; HGNC:3800; Mesoderm/mesenchyme forkhead 1; MF-1; myeloid factor-delta; Transcription factor FKH-1

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基因别名: ARA; ch; fkh-1; Fkh1; FKHL7; FOXC1; FREAC-3; FREAC3; frkhda; IGDA; IHG1; IRID1; Mf1; Mf4; RIEG3

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UniProt ID: (Human) Q12948, (Mouse) Q61572

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Entrez Gene ID: (Human) 2296, (Mouse) 17300

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功能