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FOXH1 Antibody (PA5-72644) in WB
Western blot analysis of FOXH1 in various lysates. Samples were incubated with FOXH1 polyclonal antibody (Product # PA5-72644) using a dilution of 1:1,000 followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a dilution of 1:10,000. Lysates/proteins: 20 µg per lane. Lane 1: Zebrafish lysate; Lane 2: ZF4 whole cell lysate. Predicted band size: 52 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Product Details
PA5-72644
Product Specifications
Species Reactivity
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 313-334 amino acids from the Central region of DANRE foxh1
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2718498
Target Information
FOXH1 is a member of the Forkhead-box (FOX) family of transcription factors and is the human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 plays an important role in mediating TGF-beta/activin signals through its interaction with the SMAD2-SMAD4 complex. It is expressed primarily during early development and localizes in the cytoplasm until it forms a complex with the SMAD proteins at which time it translocates to the nucleus to positively or negatively affect gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: contains winged-helix domain; etID62177.12; Fast-1; Forkhead activin signal transducer 1; Forkhead box protein H1; Schmalspur protein; transcription factor; uncle freddy; unf
Gene Aliases: fast; fast1; fe26b06; foxh1; schmalspur; SUR; wu:fe26b06; wu:fi30c08; wu:fi32b01
UniProt ID: (Zebrafish) Q9I9E1
Entrez Gene ID: (Zebrafish) 57930
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
sequence-specific DNA binding
SMAD binding
winged helix/forkhead transcription factor
negative regulation of transcription from RNA polymerase II promoter
blood vessel development
endoderm formation
mesoderm formation
heart looping
embryonic heart tube morphogenesis
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
determination of left/right symmetry
anatomical structure morphogenesis
dorsal/ventral pattern formation
specification of organ position
regulation of gene expression
notochord formation
prechordal plate formation
floor plate formation
hypothalamus development
cell differentiation
notochord development
somite rostral/caudal axis specification
floor plate development
cell migration involved in gastrulation
positive regulation of transcription from RNA polymerase II promoter
optic placode formation involved in camera-type eye formation
axial mesoderm morphogenesis
axial mesodermal cell fate specification
mesendoderm development
brain morphogenesis
Spemann organizer formation at the embryonic shield
embryonic heart tube left/right pattern formation
cellular response to cytokine stimulus
regulation of BMP signaling pathway involved in heart jogging
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