Invitrogen

FOXP3 Monoclonal Antibody (236A/E7), PE-Cyanine7, eBioscience™

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FOXP3 Antibody (25-4777-42) in Flow

Intracellular staining of normal human peripheral blood cells with Anti-Human CD25 APC (Product # 17-0259-42) and Mouse IgG1 K Isotype Control PE-Cyanine7 (Product # 25-4714-80) (left) or Anti-Human Foxp3 PE-Cyanine7 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known expression patterns, Foxp3 clone 236A/E7 stains CD4+CD25+ T cells and does not stain CD4- cells or CD4+CD25- T cells. Details: Normal human peripheral blood cells were surface stained with CD4 (clone OKT4) and CD25 (clone BC96) followed by intracellular staining with Foxp3 (clone 236A/E7) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD4- (left) and CD4+ (right) gates were used for analysis. {RE}

产品信息

25-4777-42

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

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流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human, Non-human primate, Rhesus monkey

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

236A/E7

偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2573450

产品详细信息

Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol reveals staining of the CD4+CD25bright population.

The epitope from 236A/E7 is different from that of PCH101 (Product # 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.

Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This 236A/E7 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Distinct SARS-CoV-2 specific NLRP3 and IL-1β responses in T cells of aging patients during acute COVID-19 infection.

Frontiers in immunology

Mahalingam SS,Jayaraman S,Arunkumar A,Dudley HM,Anthony DD,Shive CL,Jacobson JM,Pandiyan P

25-4777-42 was used in Flow Cytometry to reveal SARS-CoV-2-specific CD4+ and CD8+ T-cell intrinsic cytokine alterations in the individuals older than 61 and may provide new insights into dysregulated COVID-directed immune responses in the elderly.

Thu Oct 12 00:00:00 UTC 2023

In Vitro Evidence of Differential Immunoregulatory Response between MDA-MB-231 and BT-474 Breast Cancer Cells Induced by Bone Marrow-Derived Mesenchymal Stromal Cells Conditioned Medium.

Current issues in molecular biology

Arenas-Luna VM,Montesinos JJ,Cortés-Morales VA,Navarro-Betancourt JR,Peralta-Ildefonso J,Cisneros B,Hernández-Gutiérrez S

25-4777-42 was used in Flow cytometry/Cell sorting to study the effect of the conditioned medium of human bone marrow-derived-MSCs (hBM-MSC-cm) on the immunoregulatory capability of MDA-MB-231 and BT-474 breast cancer cells.

Fri Dec 30 00:00:00 UTC 2022

Modelling Graft-Versus-Host Disease in Mice Using Human Peripheral Blood Mononuclear Cells.

Bio-protocol

Haque M,Boardman DA,Lam AJ,MacDonald KN,Sanderink L,Huang Q,Fung VCW,Ivison S,Mojibian M,Levings MK

25-4777-42 was used in Flow Cytometry to describe detailed methods for how to induce xenoGvHD by injecting human peripheral blood mononuclear cells into immunodeficient NOD SCID gamma mice.

Mon Dec 05 00:00:00 UTC 2022

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

25-4777-42 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 UTC 2021

A human CD137×PD-L1 bispecific antibody promotes anti-tumor immunity via context-dependent T cell costimulation and checkpoint blockade.

Nature communications

Geuijen C,Tacken P,Wang LC,Klooster R,van Loo PF,Zhou J,Mondal A,Liu YB,Kramer A,Condamine T,Volgina A,Hendriks LJA,van der Maaden H,Rovers E,Engels S,Fransen F,den Blanken-Smit R,Zondag-van der Zande V,Basmeleh A,Bartelink W,Kulkarni A,Marissen W,Huang CY,Hall L,Harvey S,Kim S,Martinez M,O'Brien S,Moon E,Albelda S,Kanellopoulou C,Stewart S,Nastri H,Bakker ABH,Scherle P,Logtenberg T,Hollis G,de Kruif J,Huber R,Mayes PA,Throsby M

25-4777-42 was used in Flow Cytometry to describe a human CD137xPD-L1 bispecific antibody, MCLA-145, identified through functional screening of agonist- and immune checkpoint inhibitor arm combinations.

Wed Jul 21 00:00:00 UTC 2021

A conserved intratumoral regulatory T cell signature identifies 4-1BB as a pan-cancer target.

The Journal of clinical investigation

Freeman ZT,Nirschl TR,Hovelson DH,Johnston RJ,Engelhardt JJ,Selby MJ,Kochel CM,Lan RY,Zhai J,Ghasemzadeh A,Gupta A,Skaist AM,Wheelan SJ,Jiang H,Pearson AT,Snyder LA,Korman AJ,Tomlins SA,Yegnasubramanian S,Drake CG

25-4777 was used in Flow cytometry/Cell sorting to identify functional immune molecules expressed on tumor-infiltrating Tregs.

Mon Mar 02 00:00:00 UTC 2020

Sialyl-Lewis^X Glycoantigen Is Enriched on Cells with Persistent HIV Transcription during Therapy.

Cell reports

Colomb F,Giron LB,Kuri-Cervantes L,Adeniji OS,Ma T,Dweep H,Battivelli E,Verdin E,Palmer CS,Tateno H,Kossenkov AV,Roan NR,Betts MR,Abdel-Mohsen M

25-4777 was used in Flow cytometry/Cell sorting to describe a glycomic feature of HIV-infected transcriptionally active cells that not only differentiates them from their transcriptionally inactive counterparts but also may affect their trafficking abilities.

Tue Aug 04 00:00:00 UTC 2020

Phenotypic Characterization of SLe^x+ and CLA+ CD4+ T Cells.

STAR protocols

Kuri-Cervantes L,Pampena MB,Betts MR

25-4777-42 was used in Flow Cytometry to allow optimal resolution of the measured parameters while minimizing background in a 25-parameter experiment.

Fri Dec 18 00:00:00 UTC 2020

Unleashing Type-2 Dendritic Cells to Drive Protective Antitumor CD4⁺ T Cell Immunity.

Cell

Binnewies M,Mujal AM,Pollack JL,Combes AJ,Hardison EA,Barry KC,Tsui J,Ruhland MK,Kersten K,Abushawish MA,Spasic M,Giurintano JP,Chan V,Daud AI,Ha P,Ye CJ,Roberts EW,Krummel MF

25-4777 was used in Flow cytometry/Cell sorting to highlight a pathway that restrains cDC2 and whose reversal enhances CD4+ Tconv abundance and controls tumor growth.

Thu Apr 18 00:00:00 UTC 2019

Regulatory T cell features in chronic granulomatous disease.

Clinical and experimental immunology

van de Geer A,Cuadrado E,Slot MC,van Bruggen R,Amsen D,Kuijpers TW

25-4777-42 was used in Flow Cytometry to investigate whether numerical or functional deficiencies exist in Tregs of patients with chronic granulomatous disease.

Thu Aug 01 00:00:00 UTC 2019

A natural killer-dendritic cell axis defines checkpoint therapy-responsive tumor microenvironments.

Nature medicine

Barry KC,Hsu J,Broz ML,Cueto FJ,Binnewies M,Combes AJ,Nelson AE,Loo K,Kumar R,Rosenblum MD,Alvarado MD,Wolf DM,Bogunovic D,Bhardwaj N,Daud AI,Ha PK,Ryan WR,Pollack JL,Samad B,Asthana S,Chan V,Krummel MF

25-4777 was used in Flow cytometry/Cell sorting to reveal that innate immune stimulatory dendritic cells and natural killer cells cluster together as an excellent prognostic tool for T cell-directed immunotherapy and that these innate cells are necessary for enhanced T cell tumor responses, suggesting this axis as a target for new therapies.

Wed Aug 01 00:00:00 UTC 2018

Proteomic Analyses of Human Regulatory T Cells Reveal Adaptations in Signaling Pathways that Protect Cellular Identity.

Immunity

Cuadrado E,van den Biggelaar M,de Kivit S,Chen YY,Slot M,Doubal I,Meijer A,van Lier RAW,Borst J,Amsen D

25-4777 was used in Flow cytometry/Cell sorting to suggest that adaptation in signaling pathways protect Treg cell identity and present a resource for further research into Treg cell biology.

Tue May 15 00:00:00 UTC 2018

Cutting Edge: Selective Oral ROCK2 Inhibitor Reduces Clinical Scores in Patients with Psoriasis Vulgaris and Normalizes Skin Pathology via Concurrent Regulation of IL-17 and IL-10.

Journal of immunology (Baltimore, Md. : 1950)

Zanin-Zhorov A,Weiss JM,Trzeciak A,Chen W,Zhang J,Nyuydzefe MS,Arencibia C,Polimera S,Schueller O,Fuentes-Duculan J,Bonifacio KM,Kunjravia N,Cueto I,Soung J,Fleischmann RM,Kivitz A,Lebwohl M,Nunez M,Woodson J,Smith SL,West RF,Berger M,Krueger JG,Ryan JL,Waksal SD

25477742 was used in flow cytometry to find that oral administration of a selective Rho-associated kinase 2 inhibitor, KD025, reduces psoriasis area in 46% of patients with psoriasis vulgaris

Mon May 15 00:00:00 UTC 2017

Effects of conventional therapeutic interventions on the number and function of regulatory T cells.

Oncoimmunology

Roselli M,Cereda V,di Bari MG,Formica V,Spila A,Jochems C,Farsaci B,Donahue R,Gulley JL,Schlom J,Guadagni F

25-4777 was used in Flow cytometry/Cell sorting to investigate the number and function of Tregs obtained from the peripheral blood of carcinoma patients before and during chemotherapy.

Tue Oct 01 00:00:00 UTC 2013

CD4(+) regulatory T cells in a cynomolgus macaque model of Mycobacterium tuberculosis infection.

The Journal of infectious diseases

Green AM,Mattila JT,Bigbee CL,Bongers KS,Lin PL,Flynn JL

Sun Aug 15 00:00:00 UTC 2010

Early resolution of acute immune activation and induction of PD-1 in SIV-infected sooty mangabeys distinguishes nonpathogenic from pathogenic infection in rhesus macaques.

Journal of immunology (Baltimore, Md. : 1950)

Estes JD,Gordon SN,Zeng M,Chahroudi AM,Dunham RM,Staprans SI,Reilly CS,Silvestri G,Haase AT

Thu May 15 00:00:00 UTC 2008

生物信息学

蛋白别名: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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基因别名: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1

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Entrez Gene ID: (Human) 50943, (Rhesus monkey) 574303

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