Invitrogen

FZD1 Polyclonal Antibody

FZD1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

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FZD1 Antibody (PA5-47072) in IHC (F)

Immunohistochemical analysis of FZD1 in immersion fixed frozen sections of embryonic mouse intestine (13 d.p.c.). Samples were incubated in FZD1 polyclonal antibody (Product # PA5-47072) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma memb... View More

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产品信息

PA5-47072

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1 µg/mL

免疫组化（冰冻） (IHC (F))

5-15 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-1 Gln72-His248 (Met122Ile)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2610121

产品详细信息

In direct ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) Frizzled-2, rmFrizzled-4, rmFrizzled-7, and rmFrizzled-8 is observed, and less than 1% cross-reactivity with rmFrizzled-3 and rmFrizzled-6 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

靶标信息

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD1 protein contains a signal peptide, a cysteine-rich domain in the N-terminal extracellular region, 7 transmembrane domains, and a C-terminal PDZ domain-binding motif. The FZD1 transcript is expressed in various tissues.

仅用于科研。不用于诊断过程。未经明确授权不得转售。